Myosin Light Chain 2/MLC-2V Polyclonal antibody
Myosin Light Chain 2/MLC-2V Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 10906-1-AP
|Positive WB detected in||mouse heart tissue, rat heart|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10906-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
|Tested Reactivity||human, mouse, rat, zebrafish|
|Cited Reactivity||human, monkey, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Myosin Light Chain 2/MLC-2V fusion protein Ag1356|
|Full Name||myosin, light chain 2, regulatory, cardiac, slow|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||19 kDa|
|GenBank accession number||BC015821|
|Gene ID (NCBI)||4633|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MYL2 belongs to the myosin family of motor proteins. Their common features are ATP hydrolysis, actin binding, and potential for kinetic energy transduction. MYL2 stands for Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2), also known as the regulatory light chain of myosin (RLC). This isoform is distinct from those expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B), and cardiac atrial muscle (MYL7) (PMID: 21345328). Due to its tissue specificity, MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.
What is the molecular weight of MYL2?
There are two isoforms of this protein in humans that differ only slightly in length and produce proteins composed of 152 and 166 aa, which correspond to about 19 kDa (PMID: 16678204).
What is the subcellular localization of MYL2?
It is uniquely expressed in the cytoplasm of heart muscles. It colocalizes with actin cytoskeleton staining.
What is the tissue specificity of MYL2?
MYL2 is specifically expressed in ventricular cardiac tissue.
What is the function of MYL2 in cardiac tissue?
MYL2 is a contractile protein that plays a role in heart development and function. During cardiogenesis it plays an early role in cardiac contractility by promoting cardiac myofibril assembly and represents one of the earliest markers of ventricular specification (PMID 8506363).
In general, MYL2 interacts with the neck/tail region of the muscle thick filament protein myosin to regulate myosin motility and function (PMID 8316858). Its N-terminal domain is responsible for calcium and magnesium binding at their activating concentrations. This induces a functionally important conformational change (PMID: 18202317). However, the ion dissociation rate is not fast enough to modulate cardiac contractility on a beat-by-beat basis (PMIDs: 188447, 8804617). In addition, RLC function can be modulated by posttranslational modifications such as phosphorylation and deamidation in the N-terminal region. As a result, a significant change in the charge of the protein is introduced, which undoubtedly alters its interaction with the C-terminal myosin alpha-helical domain (PMID: 26074085).
What is MYL2's involvement in disease?
Diseases associated with MYL2 dysfunction include forms of familial hypertrophic cardiomyopathies, congenital fiber-type disproportion, and heart failure (PMID: 26074085).
|Product Specific Protocols|
|WB protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP||Download protocol|
|IHC protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP||Download protocol|
|IF protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP||Download protocol|
|IP protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This MLC2 antibody works very well in western blots.