Myosin Light Chain 2/MLC-2V Polyclonal antibody

Myosin Light Chain 2/MLC-2V Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10906-1-AP

Synonyms

MLC 2, MLC 2v, MLC2, MLC2V, MYL2, Myosin Light Chain 2, Myosin Light Chain 2/MLC-2V



Tested Applications

Positive WB detected inmouse heart tissue, rat heart
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10906-1-AP targets Myosin Light Chain 2/MLC-2V in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Cited Reactivityhuman, mouse, rat, monkey, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Myosin Light Chain 2/MLC-2V fusion protein Ag1356
Full Name myosin, light chain 2, regulatory, cardiac, slow
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC015821
Gene symbol MYL2
Gene ID (NCBI) 4633
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Background

MYL2 belongs to the myosin family of motor proteins. Their common features are ATP hydrolysis, actin binding, and potential for kinetic energy transduction. MYL2 stands for Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2), also known as the regulatory light chain of myosin (RLC). This isoform is distinct from those expressed in skeletal muscle (MYLPF), smooth muscle (MYL12B), and cardiac atrial muscle (MYL7) (PMID: 21345328). Due to its tissue specificity, MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

 What is the molecular weight of MYL2?

There are two isoforms of this protein in humans that differ only slightly in length and produce proteins composed of 152 and 166 aa, which correspond to about 19 kDa (PMID: 16678204). 

 What is the subcellular localization of MYL2?

 It is uniquely expressed in the cytoplasm of heart muscles. It colocalizes with actin cytoskeleton staining.

 What is the tissue specificity of MYL2?

 MYL2 is specifically expressed in ventricular cardiac tissue.

 What is the function of MYL2 in cardiac tissue?

 MYL2 is a contractile protein that plays a role in heart development and function. During cardiogenesis it plays an early role in cardiac contractility by promoting cardiac myofibril assembly and represents one of the earliest markers of ventricular specification (PMID 8506363).

 In general, MYL2 interacts with the neck/tail region of the muscle thick filament protein myosin to regulate myosin motility and function (PMID 8316858). Its N-terminal domain is responsible for calcium and magnesium binding at their activating concentrations. This induces a functionally important conformational change (PMID: 18202317). However, the ion dissociation rate is not fast enough to modulate cardiac contractility on a beat-by-beat basis (PMIDs: 188447, 8804617). In addition, RLC function can be modulated by posttranslational modifications such as phosphorylation and deamidation in the N-terminal region. As a result, a significant change in the charge of the protein is introduced, which undoubtedly alters its interaction with the C-terminal myosin alpha-helical domain (PMID: 26074085).

 What is MYL2's involvement in disease?

 Diseases associated with MYL2 dysfunction include forms of familial hypertrophic cardiomyopathies, congenital fiber-type disproportion, and heart failure (PMID: 26074085). 


Protocols

Product Specific Protocols
WB protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
IHC protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
IF protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
IP protocol for Myosin Light Chain 2/MLC-2V antibody 10906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Science

Conversion of human fibroblasts into functional cardiomyocytes by small molecules.

Authors - Nan Cao
humanIF

Cell Stem Cell

Cardiac repair in a porcine model of acute myocardial infarction with human induced pluripotent stem cell-derived cardiovascular cells.

Authors - Lei Ye
humanIF,FC

Nat Methods

Chemically defined generation of human cardiomyocytes.

Authors - Paul W Burridge
human,mouseIHC,FC

Cell Stem Cell

Lineage Reprogramming of Fibroblasts into Proliferative Induced Cardiac Progenitor Cells by Defined Factors.

Authors - Pratik A Lalit
mouseIF

Cell Stem Cell

Expandable Cardiovascular Progenitor Cells Reprogrammed from Fibroblasts.

Authors - Yu Zhang
mouseIF

Cell Res

Formative pluripotent stem cells show features of epiblast cells poised for gastrulation.

Authors - Xiaoxiao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rebecca (Verified Customer) (10-28-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: iPSC-CM
Myosin Light Chain 2/MLC-2V Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:10906-1-AP)
FH

WEI (Verified Customer) (05-11-2022)

Strong, specific band for heart tissues

  • Applications: Western Blot
  • Cell Tissue Type: Mouse Heart
FH

Irem (Verified Customer) (04-19-2022)

This antibody unspecifically recognizes at least four proteins in Jurkat cell lysate. The specific band, around 17 kDa, is so faint.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: Jurkat cells
Myosin Light Chain 2/MLC-2V Antibody Western Blot validation (1:800 dilution) in Jurkat cells (Cat no:10906-1-AP)
FH

Ning (Verified Customer) (02-20-2022)

This antibody works very well in immunofluorescence staining for iPSC-derived ventricular cardiomyocytes.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: iPSC-derived cardiomyocytes
Myosin Light Chain 2/MLC-2V Antibody Immunofluorescence validation (1:500 dilution) in iPSC-derived cardiomyocytes (Cat no:10906-1-AP)
FH

Tongbin (Verified Customer) (08-25-2020)

This MLC2 antibody works very well in western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart