MYOM1 Polyclonal antibody

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  WB analysis of mouse heart using 22084-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22084-1-AP (MYOM1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 22084-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22084-1-AP (MYOM1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 22084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 22084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 22084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 22084-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22084-1-AP (MYOM1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MYOM1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22084-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MYOM1, Myomesin 1, myomesin 1, 185kDa, Myomesin family member 1, SKELEMIN

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22084-1-AP targets MYOM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17263

  Product name: Recombinant human MYOM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1230-1589 aa of BC116183

  Sequence: TNHSTVVLVGDVFKKLQKEAEFQRQEWIRKQGPHFVEYLSWEVTGECNVLLKCKVANIKKETHIVWYKDEREISVDEKHDFKDGICTLLITEFSKKDAGIYEVILKDDRGKDKSRLKLVDEAFKELMMEVCKKIALSATDLKIQSTAEGIQLYSFVTYYVEDLKVNWSHNGSAIRYSDRVKTGVTGEQIWLQINEPTPNDKGKYVMELFDGKTGHQKTVDLSGQAYDEAYAEFQRLKQAAIAEKNRARVLGGLPDVVTIQEGKALNLTCNVWGDPPPEVSWLKNEKALASDDHCNLKFEAGRTAYFTINGVSTADSGKYGLVVKNKYGSETSDFTVSVFIPEEEARMAALESLKGGKKAK

• Full Name: myomesin 1, 185kDa
• Calculated Molecular Weight: 1685 aa, 188 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: BC116183
• Gene Symbol: MYOM1
• Gene ID (NCBI): 8736
• RRID: AB_2878989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52179
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent.