Rat Mmp9 Monoclonal antibody, PBS Only (Capture)

• 

  Sandwich ELISA standard curve of MP50079-1

  Sandwich ELISA standard curve of MP50079-1, Rat Mmp9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68695-1-PBS. Detection antibody: 68695-3-PBS. Standard: Eg0439. Range: 62.5-4000 pg/mL.

• 

  Sample test of MP50079-1

  Rat spleen and lung tissues from one adult rat (chopped in 1-2 mm pieces in 30 mL of medium) were cultured in RPMI supplemented with 10% fetal bovine serum and 50 μM β-mercaptoethanol. Aliquots of the cell culture supernates were removed, assayed for levels of rat MMP-9, and measured 5,145.7 pg/mL and 540.2 pg/mL, respectively.

• 

  Sample test of MP50079-1

  Serum of sixteen rats was measured. The MMP-9 concentration of detected samples was determined to be 37.0 ng/mL with a range of 22.7-48.2 ng/mL.

Mmp9 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68695-1-PBS

Clone No.1B1C2

Host / Isotype

Mouse / IgG1

Reactivity

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

matrix metallopeptidase 9, Matrix metalloproteinase-9

---

Product Information

68695-1-PBS targets Mmp9 as part of a matched antibody pair:

MP50079-1: 68695-1-PBS capture and 68695-3-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg0439

  Product name: Recombinant Rat MMP9 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 20-708 aa of NM_031055.2

  Sequence: APHQRQPTYVVFPRDLKTSNLTDTQLAEDYLYRYGYTRAAQMMGEKQSLRPALLMLQKQLSLPQTGELDSETLKAIRSPRCGVPDVGKFQTFEGDLKWHHHNITYWIQSYTEDLPRDVIDDSFARAFAVWSAVTPLTFTRVYGLEADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGPGIQGDAHFDDDELWSLGKGAVVPTYFGNANGAPCHFPFTFEGRSYLSCTTDGRNDGKPWCGTTADYDTDRKYGFCPSENLYTEHGNGDGKPCVFPFIFEGHSYSACTTKGRSDGYRWCATTANYDQDKLYGFCPTRADVTVTGGNSAGEMCVFPFVFLGKQYSTCTGEGRSDGRLWCATTSNFDADKKWGFCPDQGYSLFLVAAHEFGHALGLDHSSVPEALMYPMYHYHEDSPLHEDDIKGIQHLYGRGSKPDPRPPATTAAEPQPTAPPTMCPTAPPMAYPTGGPTVAPTGAPSPGPTGPPTAGPSEAPTESSTPVDNPCNVDVFDAIADIQGALHFFKDGRYWKFSNHGGSQLQGPFLIARTWPALPAKLNSAFEDPQSKKIFFFSGRKMWVYTGQSVLGPRSLDKLGLGSEVTLVTGLLPRRGGKALLISRERIWKFDLKSQKVDPQSVTRLDNEFSGVPWNSHNVFHYQDKAYFCHDKYFWRVSFHNRVNQVDHVAYVTYDLLQCP

• Full Name: matrix metallopeptidase 9
• Calculated Molecular Weight: 79kd
• GenBank Accession Number: NM_031055.2
• Gene Symbol: Mmp9
• Gene ID (NCBI): 81687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P50282
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.