Mouse Adiponectin ELISA Kit


6.9 pg/mL


31.25-2000 pg/mL



Cat no : KE10044


30kDa, Acdc, Acrp30, adipo, Adipoq, apM1, APN, GBP28

Product Information

KE10044 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Adiponectin ELISA kit is to be used to detect and quantify protein levels of endogenous Adiponectin. The assay recognizes mouse Adiponectin. An antibody specific for Adiponectin has been pre-coated onto the microwells. The Adiponectin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Adiponectin is added to detect the captured Adiponectin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

Product name Mouse Adiponectin ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell Culture Supernates, Tissue homogenates,
Assay type Sandwich
Sensitivity 6.9 pg/mL
Range 31.25-2000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 11450


Sample TypeAverageRange
Mouse serum102%91%-122%
Mouse fat tissue Homogenates96%82%-106%
Cell Culture Supernates 93%77%-104%


Samplenmean ( pg/mL)SDCV%


Samplenmean ( pg/mL)SDCV%

Background Information

Adiponectin, also known as Acrp30, AdipoQ, and GBP28, is an adipocyte-secreted protein that circulates in high concentrations in the serum and acts to increase insulin sensitivity. Adiponectin modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. The serum concentration of adiponectin decreases in obese patients, opposite to other adipocytokines. In recent studies, serum adiponectin level progressively decreased in patients according to the severity of obesity, insulin resistance, diabetes mellitus and cardiovascular diseases. Low serum adiponectin level may be a predictive factor for type 2 diabetes mellitus (T2DM) and other cardiovascular diseases.


Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms 30kDa, Acdc, Acrp30, adipo, Adipoq, apM1, APN, GBP28


SpeciesSample TypeTitle

J Cachexia Sarcopenia Muscle

Pim1 knockout alleviates sarcopenia in aging mice via reducing adipogenic differentiation of PDGFRα+ mesenchymal progenitors.

Authors - Guo-Kai Shang

Food Res Int

Whey protein hydrolysate alleviated atherosclerosis and hepatic steatosis by regulating lipid metabolism in apoE-/- mice fed a Western diet.

Authors - Kai Wang
mousecell culture supernatants

Hum Cell

Enzyme-digested Colla Corii Asini (E'jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes.

Authors - Li Xiao

Diabetes Metab Syndr Obes

Deep Sea Water Inhibited Pancreatic β-Cell Apoptosis and Regulated Glucose Homeostasis by Affecting Lipid Metabolism in Db/Db Mice

Authors - Mengxia Shi
mousecell culture supernatants

Biochem Biophys Res Commun

Types of cell culture inserts affect cell crosstalk between co-cultured macrophages and adipocytes

Authors - Li Xiao

Int J Mol Sci

Oral Administration of Lipopolysaccharide Enhances Insulin Signaling-Related Factors in the KK/Ay Mouse Model of Type 2 Diabetes Mellitus

Authors - Kazushi Yamamoto