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Recombinant Mouse CD13 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3592

Validation Data Gallery

  • Purity of Recombinant Mouse CD13 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD13 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CD13 protein Asp66-Ser966 (Accession# P97449) with a rabbit IgG Fc tag at the C-terminus.
GeneID 16790
Accession P97449
PredictedSize 129.4 kDa
SDS-PAGE 120-150 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD13, also known as aminopeptidase N or membrane alanyl aminopeptidase, is a type II membrane 150kDa metalloprotease with an extracellular oriented catalytic domain. It is a seahorse-shaped molecule and usually forms a head-to-head homodimer by means of hydrophobic interactions. Each monomeric molecule of CD13 possesses a 7-domain organization, which is characteristic of M1 metallopeptidases. CD13 has been termed a “moonlighting enzyme” because of its multiple functions.

References:

1.Wong AH. et al. (2012). J. Biol. Chem 287: 36804–36813.
2.Chen L. et al. (2012). Proc. Natl. Acad. Sci. U. S. A 109: 17966–17971.
3.Sjöström H. et al. (2000). Adv. Exp. Med. Biol 477: 25–34.
4.Mina-Osorio P. (2008). Trends Mol. Med 14: 361–371.