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Recombinant Mouse CD163 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0794

Validation Data Gallery

  • Purity of Recombinant Mouse CD163 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CD163 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression CHO-derived Mouse CD163 protein Val39-Thr1045 (Accession# Q2VLH6-1) with a His tag at the C-terminus.
GeneID 93671
Accession Q2VLH6-1
PredictedSize 109.5 kDa
SDS-PAGE 110-130 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily. It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages. CD163 mediates endocytosis of haptoglobin-haemoglobin complexes. The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

References:

1. S K Law, et al. (1993) Eur J Immunol. 23(9):2320-5.
2. M Kristiansen, et al. (2001) Nature. 409(6817):198-201.
3. Anders Etzerodt, et al. (2013) Antioxid Redox Signal. 18(17):2352-63.
4. H J Møller, et al. (2002) Scand J Clin Lab Invest Suppl. 237:29-33.