Recombinant Mouse CD43 protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1395

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Validation Data Gallery

• 

  Purity of Recombinant Mouse CD43 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse CD43 protein Asp20-Gly248 (Accession# P15702) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 20737
• Accession: P15702
• PredictedSize: 48.8 kDa
• SDS-PAGE: kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage.CD43 has also been suggested to be present in colon adenomas and colon carcinomas. CD43 also mediates leukocyte homing to inflammatory sites through binding to E-selectin expressed in endothelial cells from inflamed tissues.

References:

1. Alisson-Silva F. et al. (2019) Sci Rep. 9(1):8628.
2. Batdorf BH. et al. (2017) Tumour Biol. 23(4):193-201.
3. Matsumoto M. et al. (2008) J Immunol. 181(5):3628-35.