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Recombinant Mouse CXCL2 protein (rFc Tag) (HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2239

Validation Data Gallery

  • Purity of Recombinant Mouse CXCL2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse CXCL2 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse CXCL2 was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse CXCL2 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse CXCL2 protein Ala28-Asn100 (Accession# P10889) with a rabbit IgG Fc tag at the N-terminus.
GeneID 20310
Accession P10889
PredictedSize 35.0 kDa
SDS-PAGE 34-38 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CXCL2, also known as macrophage inflammatory protein 2-alpha (Mip2-alpha), is a member of CXC chemokine family that binds to CXC chemokine receptor 2 (CXCR2). CXCL2 is mainly produced by macrophages, endothelial cells, epithelial cells and tumor cells. CXCL2 plays important roles in various biological progresses such as angiogenesis, inflammation, immune response and cancer biological behaviors. Under inflammatory conditions in CNS, microglia and astrocytes may secrete CXCL2 as well as other chemokines, which induces chemotaxis and infiltration of circulatory neutrophils. Indeed, previous studies suggest that CXCL2 is involved in Alzheimer disease, multiple sclerosis and brain ischemia.

References:

1. Vansaun MN. et al. (2013). PLoS One. 8(9):e73054.
2. Jablonska J. et al. (2014). Int J Cancer. 134:1346-58.
3. Oue E. et al. (2012). Biochem Biophys Res Commun. 424:456-461.
4. Haraguchi K. et al. (2012). J. Neurosci.32:3931-3941.
5. L. Bozoyan. et al. (2015). J. Neuroinflammation. 12:173.
6. Hosking MP. et al. (2010). PLoS One. 5(6):e11340.