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Recombinant Mouse Erythropoietin/EPO protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0265

Validation Data Gallery

  • Purity of Recombinant Mouse Erythropoietin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Erythropoietin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Erythropoietin protein Ala27-Arg192 (Accession# P07321) with a His tag at the C-terminus.
GeneID 13856
Accession P07321
PredictedSize 22.7 kDa
SDS-PAGE 32-45 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Erythropoietin (EPO) is a glycoprotein hormone that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults, and and the secreted protein will travel through the blood stream to reach to the bone marrow to stimulate hematopoietic stem cell differentiation to RBC. EPO binds to the cognate EPO receptor (EPOR) on erythroid progenitor cells, thus preventing apoptosis and stimulating their differentiation and maturation into erythrocyte. However, EPO protein and its receptors have also been shown to be cytoprotective in extra-hematopoietic tissues including the retina tissue. A complete lack of erythropoietin causes embryonic lethal anemia in mice. The conditional inactivation of erythropoietin in adult mice results in a chronic, normocytic and normochromic anemia. 

References:

1. Chateauvieux S. et al.(2011) Biochem Pharmacol. 15;82(10):1291-303.
2. Caprara C. et al.(2014) Mol Vis. 20:307-24.
3. Brines M. et al. (2004) Proc Natl Acad Sci U S A. 101(41):14907-12.
4. Dis Model Mech. et al. (2010) Dis Model Mech. 3(11-12):763-72.