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Recombinant Mouse FGF-21 protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0259

Validation Data Gallery

  • Purity of Recombinant Mouse FGF-21 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse FGF-21 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse FGF-21 protein Ala29-Ser210 (Accession# Q9JJN1) with a His tag at the C-terminus.
GeneID 56636
Accession Q9JJN1
PredictedSize 23.7 kDa
SDS-PAGE 25-27 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is expressed across several tissues, predominantly in liver and adipocyte tissues, and, to a lower extent, in the pancreas, skeletal muscle, heart, kidneys and testes. FGF21 plays a role in regulating adaptation to various metabolic abnormalities. In the liver, FGF21 plays an important role in the regulation of fatty acid oxidation both in the fasted state and in mice consuming a high-fat, low-carbohydrate ketogenic diet. FGF21 also regulates fatty acid metabolism in mice consuming a diet that promotes hepatic lipotoxicity.

References:

1. Fisher FM. et al.(2016).  Annu Rev Physiol. 78:223-41.
2. Strowski MZ. et al. (2017). Horm Mol Biol Clin Investig. 30(2).
3. Nishimura T. et al.(2000). Biochim Biophys Acta.1492(1):203-6.
4. Kharitonenkov A. et al. (2009). Curr Opin Investig Drugs.10(4):359-64.