Recombinant Mouse G-CSF protein (His Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0256

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Validation Data Gallery

• 

  Purity of Recombinant Mouse G-CSF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Mouse G-CSF protein Val31-Ala208 (Accession# P09920) with a His tag at the C-terminus.
• GeneID: 12985
• Accession: P09920
• PredictedSize: 22.8 kDa
• SDS-PAGE: 24-32 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT) and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.

References:

1. Panopoulos AD. et al. (2008). Cytokine. 42(3):277-88.
2. Zhao SQ. et al. (2015). Zhongguo Shi Yan Xue Ye Xue Za Zhi. 23(3):871-7.
3. Sheridan WP. et al.(1989). Lancet. 2(8668):891-5.
4. Sheridan WP. et al.(1992). 339(8794):640-4.
5. Yamada M. et al. (2002). 92(5):1789-94.
6. Mooren FC. Et. Al.(2012). 113(7):1082-90.