Mouse IL-6 ELISA Kit
Cat no : KE10007
Synonyms
Il 6, Il6, IL-6, interleukin 6, Interleukin-6
Validation Data Gallery
Product Information
KE10007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-6. The assay recognizes mouse IL-6. An antibody specific for mouse IL-6 has been pre-coated onto the microwells. The mouse IL-6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-6 is added to detect the captured mouse IL-6 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Mouse IL-6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 3.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16193 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 99% | 75%-119% |
| Cell culture supernatants | 108% | 96%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 133.3 | 6.1 | 4.5 |
| 2 | 20 | 204.3 | 7.6 | 3.7 |
| 3 | 20 | 778.1 | 52.7 | 6.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 142.3 | 11.6 | 8.2 |
| 2 | 24 | 223.0 | 18.8 | 8.4 |
| 3 | 24 | 827.3 | 81.2 | 9.8 |
Background Information
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Il 6, Il6, IL-6, interleukin 6, Interleukin-6 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Adv Mater Biomimetic Immunosuppressive Exosomes that Inhibit Cytokine Storms Contribute to the Alleviation of Sepsis. | ||
Bioact Mater Turning foes to friends: Advanced "in situ nanovaccine" with dual immunoregulation for enhanced immunotherapy of metastatic triple-negative breast cancer | ||
ACS Nano A Dual-Targeted Metal-Organic Framework Based Nanoplatform for the Treatment of Rheumatoid Arthritis by Restoring the Macrophage Niche | ||
Adv Sci (Weinh) Exosomal miR-181d-5p Derived from Rapamycin-Conditioned MDSC Alleviated Allograft Rejection by Targeting KLF6 | ||
Adv Sci (Weinh) Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury | ||
Bone Res Osteopontin deficiency promotes cartilaginous endplate degeneration by enhancing the NF-κB signaling to recruit macrophages and activate the NLRP3 inflammasome |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chintan (Verified Customer) (06-17-2019) | Primary microglia cells were isolated from mouse brain and treated with LPS. IL-6 concentrations were analysed using this kit. It worked as expected.
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FH Joanna (Verified Customer) (06-07-2019) | The ELISA kit was used in order to detect changes between control and LPA (1µM) treated microglia cells. The kit was easy to use, including a very helpful and informative brochure. Results were consistent and I highly recommend this kit.
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FH Joanna (Verified Customer) (06-07-2019) | The ELISA was used in order to detect changes in IL6 levels in control and LPA treated microglia cells. The kit was easy to use, including a detailed and very informative brochure. The results were consistent and this kit is highly recommended.
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FH Chintan (Verified Customer) (06-03-2019) | This kit was used to estimate IL-6 levels in the cell culture medium of microglia cells. After stimulation with LPS, we observed elevated IL-6 production and excretion by microglia. This kit is easy to use. Standard curve and sample /positive control analysis worked perfectly.
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