Mouse IL-6 ELISA Kit

Sensitivity

3.8 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Mouse

Cat no : KE10007

Synonyms

Il 6, Il6, IL-6, interleukin 6, Interleukin-6



Product Information

KE10007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL6. The assay recognizes mouse IL6. An antibody specific for mouse IL6 has been pre-coated onto the microwells. The mouse IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL6 is added to detect the captured mouse IL6 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Mouse IL-6 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 3.8 pg/mL
Range 15.6-1000 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Mouse serum99%75%-119%
Cell culture supernatants108%96%-123%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120133.36.14.5
220204.37.63.7
320778.152.76.8

InterAssay

Samplenmean ( pg/mL)SDCV%
124142.311.68.2
224223.018.88.4
324827.381.29.8

Background Information

Interleukin-6 (Il-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. Il-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. Il-6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Il-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. Il-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, Il-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Il 6, Il6, IL-6, interleukin 6, Interleukin-6

Publications

SpeciesSample TypeTitle

Int J Mol Sci

Network Pharmacology-Based Investigation of Protective Mechanism of Aster tataricus on Lipopolysaccharide-Induced Acute Lung Injury.

Authors - Yijun Chen
mouse

ACS Appl Mater Interfaces

Surface-Functionalized Modified Copper Sulfide Nanoparticles Enhance Checkpoint Blockade Tumor Immunotherapy by Photothermal Therapy and Antigen Capturing.

Authors - Ruoping Wang
mousetissue homogenates

J Infect Dis

TIPE2 suppresses Pseudomonas aeruginosa keratitis by inhibiting NF-κB signaling and the infiltration of inflammatory cells.

Authors - Qun Wang
mouseserum

Neuropharmacology

Identification of a microglial activation-dependent antidepressant effect of amphotericin B liposome.

Authors - Minhui Gao
mousecell culture supernatants

Cells

Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

Authors - Takayuki Inoue
mouseplasma

Biomed Pharmacother

Chaihu-Shugan-San and absorbed meranzin hydrate induce anti-atherosclerosis and behavioral improvements in high-fat diet ApoE-/- mice via anti-inflammatory and BDNF-TrkB pathway.

Authors - Lan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chintan (Verified Customer) (06-17-2019)

Primary microglia cells were isolated from mouse brain and treated with LPS. IL-6 concentrations were analysed using this kit. It worked as expected.

  • Applications: Western Blot,Cell culture,
  • Cell Tissue Type: primary microglia cells
FH

Joanna (Verified Customer) (06-07-2019)

The ELISA kit was used in order to detect changes between control and LPA (1µM) treated microglia cells. The kit was easy to use, including a very helpful and informative brochure. Results were consistent and I highly recommend this kit.

  • Applications: Cell culture,
  • Cell Tissue Type: BV2 microglia cells
FH

Joanna (Verified Customer) (06-07-2019)

The ELISA was used in order to detect changes in IL6 levels in control and LPA treated microglia cells. The kit was easy to use, including a detailed and very informative brochure. The results were consistent and this kit is highly recommended.

  • Applications: Cell culture,
  • Cell Tissue Type: BV2 microglia cells
FH

Chintan (Verified Customer) (06-03-2019)

This kit was used to estimate IL-6 levels in the cell culture medium of microglia cells. After stimulation with LPS, we observed elevated IL-6 production and excretion by microglia. This kit is easy to use. Standard curve and sample /positive control analysis worked perfectly.

  • Applications: Cell culture,
  • Cell Tissue Type: Mouse microglia