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Recombinant Mouse MIF protein (His Tag)

Species

Mouse

Purity

>85 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0574

Validation Data Gallery

  • Purity of Recombinant Mouse MIF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse MIF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >85 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse MIF protein Pro2-Ala115 (Accession# P34884) with a His tag at the C-terminus.
GeneID 17319
Accession P34884
PredictedSize 13.2 kDa
SDS-PAGE 16-20 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Macrophage migration inhibitory factor (MIF) is an upstream regulator of the innate and adaptive immune response that is widely expressed and that exhibits chemokine-like activities. MIF play a role in the pathogenesis of inflammatory diseases, such as atherosclerosis, rheumatoid arthritis, sepsis, asthma, and acute respiratory distress syndrome. MIF is expressed by the ischemic myocardium and that it may exhibit an overall cardioprotective role during I/R injury. MIF regulates inflammatory processes by recruiting immune cells, amplifying cytokine production, inhibiting apoptosis and counter-regulating the immunosuppressive action of glucocorticoids.

References:

1. Calandra T. et al. (2003). Nat Rev Immunol. 3(10):791-800.
2. Koga K. et al.(2011). Antioxid Redox Signal. 14(7):1191-202.
3. Qi D. et al.(2009). J Clin Invest. 119(12):3807-16.
4. Stoppe C. et al.(2012). Mol Med.18:843-50.
5. Rajasekaran D. et al.(2014). 28(11):4961-71.
6. Bernhagen J. et al. (2007). 13(5):587-96.