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Recombinant Mouse Podoplanin/PDPN protein (rFc Tag)(HPLC verified)

Species

Mouse

Purity

>90 %, SDS-PAGE
>90%, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg2861

Validation Data Gallery

  • Purity of Recombinant Mouse Podoplanin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Podoplanin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse Podoplanin/PDPN was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse Podoplanin/PDPN was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90%, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse Podoplanin protein Gln21-Lys133 (Accession# Q62011) with a rabbit IgG Fc tag at the C-terminus.
GeneID 14726
Accession Q62011
PredictedSize 37.9 kDa
SDS-PAGE 43-63 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Podoplanin (PDPN) is a transmembrane glycoprotein that plays a role in a variety of biological processes including regulation of blood-lymphatic vessel development, cerebral vascular pattern, and integrity, cell motility, tumorigenesis and metastasis, and mediates tumor cell-induced platelet aggregation in different cancer types. PDPN homologs are found in humans, mice, rats, dogs, and hamsters and are relatively conserved among species.

References:

1. Ukaji T, et al . Cancer Sci. (2021). 112(6):2299-2313.                 
2. Krishnan H, et al. J Biol Chem. (2013). 288(17):12215-21.                                                             
3. Wang X, et al. Cancer Gene Ther. (2023). 30(2):345-357.                                                        
4.Astarita JL, et al. Front Immunol. (2012). 3:283.