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Recombinant Mouse SOST/Sclerostin protein (rFc Tag)

Species

Mouse

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3294

Validation Data Gallery

  • Purity of Recombinant Mouse SOST was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse SOST was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Mouse SOST protein Gln24-Tyr211 (Accession# Q99P68) with a rabbit IgG Fc tag at the N-terminus.
GeneID 74499
Accession Q99P68
PredictedSize 48.3 kDa
SDS-PAGE 50-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Sclerostin (Sost) is a potent negative regulator of bone mass, where it normally inhibits Wnt signaling through interactions with low‐density lipoprotein receptor‐related protein (LRP) 5/6 co‐receptors. Expression of the gene product sclerostin in bone is restricted to osteocytes. In the absence of Sost protein, patients develop two types of hyperostosis, sclerosteosis and van Buchem disease. Sclerosteosis and Van Buchem disease are two closely related bone disorders characterized by progressive bone thickening due to increased bone formation. Sclerosteosis is associated with mutations in the SOST gene and Van Buchem disease with a 52 kb deletion downstream of the SOST gene that probably affects transcription of the gene.

References:

1.van Bezooijen, et al. (2005) Biochemistry.16(3):319-327. 
2.Chang Jiun C, et al. (2018) Biochemistry.33(6):1105-1113. 
3.Sun Lisha, et al. (2022) Biochemistry.21(1):228.