Myogenin Monoclonal antibody

Myogenin Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig and More (5)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

15

CloneNo.

1C10B9

Cat no : 67082-1-Ig

Synonyms

bHLHc3, Myf 4, MYF4, MYOG, Myogenic factor 4, MYOGENIN, myogenin (myogenic factor 4)

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Validation Data Gallery

• 

  WB analysis of human skeletal muscle using 67082-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 67082-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67082-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67082-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67082-1-Ig (Myogenin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue, human heart tissue, pig heart tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IF: See 3 publications below

Product Information

67082-1-Ig targets Myogenin in WB, IF, ELISA applications and shows reactivity with Human, Rat, Pig samples.

• Tested Reactivity: Human, Rat, Pig
• Cited Reactivity: human, mouse, rabbit, bovine, goat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Myogenin fusion protein Ag6414
• Full Name: myogenin (myogenic factor 4)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC053899
• Gene Symbol: Myogenin
• Gene ID (NCBI): 4656
• RRID: AB_2882390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOG (encoded Myogenin protein) is a muscle-specific transcription factor which belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit and muscle atrophy(PMID: 30315160). It can be initiate the myogenic program in myoblasts, fiblasts and veritey of other cell types. Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.

Publications

Species	Application	Title
mouse	WB	J Cachexia Sarcopenia Muscle; circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.; Authors - Junyu Yan
human	WB	Mol Ther Nucleic Acids; Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.; Authors - Chen Yang
mouse	WB	Int J Mol Sci; G6PD Deficiency Is Crucial for Insulin Signaling Activation in Skeletal Muscle.; Authors - Aiwen Jiang
mouse	WB	Front Cell Dev Biol; Phosphorylation of Dynamin-Related Protein 1 (DRP1) Regulates Mitochondrial Dynamics and Skeletal Muscle Wasting in Cancer Cachexia.; Authors - Xiangyu Mao
goat	WB	FASEB J; Circular RNA circUSP13 sponges miR-29c to promote differentiation and inhibit apoptosis of goat myoblasts by targeting IGF1.; Authors - Zhen Zhang
	WB	FASEB J; MicroRNA profiling reveals miR-145-5p inhibits goat myoblast differentiation by targeting the coding domain sequence of USP13.; Authors - Zhen Zhang