NACA Polyclonal antibody

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  WB analysis of HeLa using 32235-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 32235-1-AP (NACA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 32235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 32235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32235-1-AP (NACA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NACA Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32235-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Alpha NAC, Hom s 2, HSD48, NAC alpha, NACA1

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Tested Applications

• Positive WB detected in: HeLa cells, U-87 MG cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32235-1-AP targets NACA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NACA fusion protein Ag37704
• Full Name: nascent polypeptide-associated complex alpha subunit
• Calculated Molecular Weight: 215 aa, 23 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC106041
• Gene Symbol: NACA
• Gene ID (NCBI): 4666
• RRID: AB_3670227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q13765
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NACA (NAC-alpha), or Nascent polypeptide-associated complex subunit alpha, is a component of the nascent polypeptide-associated complex (NAC). NACA is a key subunit of the NAC complex that assists in the proper folding of newly synthesized proteins and prevents the formation of harmful protein aggregates, playing a vital role in cellular protein quality control and stress response mechanisms. The calculated MW of NACA is 23 kDa, and the observed MW is 35 kDa, which is due to phosphorylation modification (PMID: 30948508, 36107769).