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NLRP3 Polyclonal antibody

NLRP3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 19771-1-AP

Synonyms

AGTAVPRL, AII, AII/AVP, AVP, C1orf7, Caterpiller protein 1.1, CIAS1, CLR1.1, Cryopyrin, FCAS, FCU, MWS, NALP3, NLRP3, PYPAF1



Tested Applications

Positive WB detected inRaji cells, THP-1 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19771-1-AP targets NLRP3 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, goat, chicken, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name NLR family, pyrin domain containing 3
Calculated Molecular Weight 118 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberNM_001127461
Gene Symbol NLRP3
Gene ID (NCBI) 114548
RRIDAB_10646484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NALP3, also named C1orf7, CIAS1, and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which is also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which is also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3.

Protocols

Product Specific Protocols
WB protocol for NLRP3 antibody 19771-1-APDownload protocol
IHC protocol for NLRP3 antibody 19771-1-APDownload protocol
IF protocol for NLRP3 antibody 19771-1-APDownload protocol
FC protocol for NLRP3 antibody 19771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
humanWB,IF

Sci Adv

Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.

Authors - Xinwen Zhang
WB

Nat Commun

UAF1 deubiquitinase complexes facilitate NLRP3 inflammasome activation by promoting NLRP3 expression.

Authors - Hui Song
WB

Nat Commun

TRIM28 SUMOylates and stabilizes NLRP3 to facilitate inflammasome activation.

Authors - Ying Qin
IHC

Nat Commun

NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis.

Authors - Nour Ershaid
humanWB

Research (Wash D C)

Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis

Authors - Lang Linghu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain