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NLRP3 Polyclonal antibody
NLRP3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 19771-1-AP
|Positive WB detected in
|Raji cells, THP-1 cells
|Positive IHC detected in
|human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, Check data in validation data gallery.
19771-1-AP targets NLRP3 in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human samples.
|human, goat, chicken, pig, bovine
|Host / Isotype
|Rabbit / IgG
|NLR family, pyrin domain containing 3
|Calculated Molecular Weight
|Observed Molecular Weight
|GenBank Accession Number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
NALP3, also named C1orf7, CIAS1, and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which is also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which is also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3.
|Product Specific Protocols
|WB protocol for NLRP3 antibody 19771-1-AP
|IHC protocol for NLRP3 antibody 19771-1-AP
|IF protocol for NLRP3 antibody 19771-1-AP
|FC protocol for NLRP3 antibody 19771-1-AP
|Click here to view our Standard Protocols
An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation
Oligodendroglial glycolytic stress triggers inflammasome activation and neuropathology in Alzheimer's disease.
UAF1 deubiquitinase complexes facilitate NLRP3 inflammasome activation by promoting NLRP3 expression.
TRIM28 SUMOylates and stabilizes NLRP3 to facilitate inflammasome activation.
NLRP3 inflammasome in fibroblasts links tissue damage with inflammation in breast cancer progression and metastasis.
Research (Wash D C)
Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Macarena Lucia (Verified Customer) (10-17-2022)
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.