|Positive WB detected in||THP-1 cells, Raji cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19771-1-AP targets NLRP3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||cow, human, swine|
|Host / Isotype||Rabbit / IgG|
|Full Name||NLR family, pyrin domain containing 3|
|Calculated molecular weight||118 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||NM_001127461|
|Gene ID (NCBI)||114548|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NALP3, also named as C1orf7, CIAS1 and PYPAF1, belongs to the NLRP family. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3.
Detection of Exosomal PD-L1 RNA in Saliva of Patients With Periodontitis.
J Clin Neurosci
MicroRNA-135b alleviates MPP+-mediated Parkinson's disease in in vitro model through suppressing FoxO1-induced NLRP3 inflammasome and pyroptosis.
Cell Death Dis
LXRα promotes cell metastasis by regulating the NLRP3 inflammasome in renal cell carcinoma.
Cell Death Dis
Endothelial cell pyroptosis plays an important role in Kawasaki disease via HMGB1/RAGE/cathespin B signaling pathway and NLRP3 inflammasome activation.
Biochim Biophys Acta
Thioredoxin-interacting protein mediates NALP3 inflammasome activation in podocytes during diabetic nephropathy.
Int J Mol Med
MCMV triggers ROS/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.