Tested Applications
| Positive WB detected in | THP-1 cells, Raji cells, LPS treated THP-1 cells |
| Positive IP detected in | THP-1 cells |
| Positive IF/ICC detected in | RAW 264.7 cells, HepG2 cells |
| Positive FC (Intra) detected in | THP-1 cells |
NLRP3 expression is relatively low in normal tissues(PMID: 33538177, 35676979, 25524927, 24166187)
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19771-1-AP targets NLRP3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, monkey, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | NLR family, pyrin domain containing 3 |
| Calculated Molecular Weight | 118 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | NM_001127461 |
| Gene Symbol | NLRP3 |
| Gene ID (NCBI) | 114548 |
| RRID | AB_10646484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96P20 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NALP3, also named C1orf7, CIAS1, and PYPAF1, belongs to the NLRP family. NLRP3, a key and eponymous component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. NALP3 may function as an inducer of apoptosis. It interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling.NALP3 inhibits TNF-alpha-induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. NALP3 activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) which is also known as familial cold urticaria. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) which is urticaria-deafness-amyloidosis syndrome. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) which is also known as neonatal onset multisystem inflammatory disease (NOMID). The antibody recognizes the C-term of NALP3.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NLRP3 antibody 19771-1-AP | Download protocol |
| IF protocol for NLRP3 antibody 19771-1-AP | Download protocol |
| IP protocol for NLRP3 antibody 19771-1-AP | Download protocol |
| WB protocol for NLRP3 antibody 19771-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-NLRP3 antibody (Cat# 19771-1-AP) has 734 total citations published across over 60 journals, including Nature Communications, Science Advances, Cell Death & Disease, Journal of Advanced Research, Redox Biology, Molecular Cell, Phytomedicine, Frontiers in Immunology, and Journal of Ethnopharmacology. Key research fields include pyroptosis and inflammasome biology, atherosclerosis, neurodegeneration, cancer, liver fibrosis, renal injury, cardiovascular disease, environmental toxicology, traditional Chinese medicine, and nanomedicine.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Pulsed electromagnetic fields inhibit atherosclerosis by regulating pyroptosis through membrane tension-mediated mechanosensitive channels | ||
Gastroenterology Protein Misfolding Enteropathy Predicts and Prognosticates Neurodegenerative Disease Years Before Symptom Onset. | ||
Adv Mater Functionalized Nanozyme Microcapsules Targeting Deafness Prevention via Mitochondrial Homeostasis Remodeling | ||
Adv Mater Multifunctional Copper-Phenolic Nanopills Achieve Comprehensive Polyamines Depletion to Provoke Enhanced Pyroptosis and Cuproptosis for Cancer Immunotherapy | ||
Cell Stem Cell Niche inflammatory signals control oscillating mammary regeneration and protect stem cells from cytotoxic stress |
Reviews
What customers say
Most reviewers report positive results. For Western blot, users observed clear bands in rat and mouse tissues, with one noting that following Proteintech's antibody-specific protocol after contacting technical support resolved initial difficulties. For IHC on non-human primate samples, one user found it produced the cleanest signal compared to other NLRP3 antibodies, while another reported nonspecific staining in kidney and liver at the tested concentration and was re-evaluating at lower dilutions. Overall, the antibody performs well in WB across species, with mixed IHC results in NHP tissues depending on optimization.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jillian (Verified Customer) (05-27-2025) | We tried out three different NLRP3 Rb antibodies on NHP samples and this was the cleanest signal we found. Will be ordering again for future projects.
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Avelina (Verified Customer) (04-14-2025) | We titrated this antibody for use in NHP in kidney and liver/gallbladder and staining appears nonspecific. Will be repeating testing at lower concentrations to determine specificity.
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Anita (Verified Customer) (09-20-2024) | Initially had trouble getting the WB to work. Reached out to technical support. Had not realised that Proteintech actually develops protocols that are specific for the antibody. Followed the tech support recommendations as well as WB protocol and the WB worked out better than expected.
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Macarena Lucia (Verified Customer) (10-17-2022) | clear band.
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Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
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