Nanog Monoclonal antibody

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  WB analysis using 67255-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67255-1-Ig (Nanog antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Nanog Monoclonal Antibody for WB, ELISA

Cat No. 67255-1-Ig

Clone No.3A2E1

Publications(17)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, ELISA

Synonyms

hNanog, 3A2E1

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Tested Applications

• Positive WB detected in: HT-29 cells, HuH-7 cells, MDA-MB-231 cells, MCF-7 cells, T-47D cells, NCCIT cells, JAR cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IF: See 1 publications below

Product Information

67255-1-Ig targets Nanog in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Nanog fusion protein Ag21364
• Full Name: Nanog homeobox
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC160187
• Gene Symbol: Nanog
• Gene ID (NCBI): 79923
• RRID: AB_2882529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H9S0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma; Authors - Yongzhi Zhao
human	WB	J Pineal Res; Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis; Authors - Xinyue Luo
human	WB,IF	Dent Mater; Microspheres of stem cells from human exfoliated deciduous teeth exhibit superior pulp regeneration capacity; Authors - Jianzhao Ding
human	WB	iScience; Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.; Authors - Xin Chen
human	WB	Biomed Pharmacother; Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.; Authors - Ling Gao
human	WB	Med Sci Monit; PMID: 31760402; Authors - Xueni Liu