NBR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NBR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

40

Cat no : 16004-1-AP

Synonyms

1A1 3B, 1A13B, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B

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Validation Data Gallery

• 

  WB analysis using 16004-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16004-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 16004-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16004-1-AP

  IP Result of anti-NBR1 (IP:16004-1-AP, 4ug; Detection:16004-1-AP 1:800) with HeLa cells lysate 2500ug.

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  IHC staining of mouse heart using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16004-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NBR1 antibody (16004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 16004-1-AP

  1X10^6 HeLa cells were stained with 0.2ug NBR1 antibody (16004-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, COS-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse heart tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 6 publications below
• WB: See 25 publications below
• IHC: See 10 publications below
• IF: See 14 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Product Information

16004-1-AP targets NBR1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NBR1 fusion protein Ag8652
• Full Name: neighbor of BRCA1 gene 1
• Calculated molecular weight: 966 aa, 107 kDa
• Observed molecular weight: 140 kDa
• GenBank accession number: BC009808
• Gene symbol: NBR1
• Gene ID (NCBI): 4077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).

Publications

Species	Application	Title
human	WB	Cell; Lack of Neuronal IFN-β-IFNAR Causes Lewy Body- and Parkinson's Disease-like Dementia.; Authors - Patrick Ejlerskov
mouse	WB, IHC	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter
human	WB	Autophagy; TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression; Authors - Tai-Mei Zhang
human	WB,IF	Autophagy; Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules; Authors - Cuiwei Yang ; KD Validated
human	IF	Autophagy; Identification of a lung cancer cell line deficient in atg7-dependent autophagy.; Authors - Jonathan Mandelbaum
human	IF	Autophagy; Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.; Authors - Marcus Nordgren

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