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CoraLite®594-conjugated NBR1 Polyclonal antibody

NBR1 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-16004

Synonyms

1A1 3B, 1A13B, FLJ55359, FLJ98272, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16004-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-16004 targets NBR1 in IF applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NBR1 fusion protein Ag8652
Full Name neighbor of BRCA1 gene 1
Calculated molecular weight 966 aa, 107 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC009808
Gene symbol NBR1
Gene ID (NCBI) 4077
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).

Protocols

Product Specific Protocols
IF protocol for CL594 NBR1 antibody CL594-16004Download protocol
Standard Protocols
Click here to view our Standard Protocols