ND4 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85098-1-RR (same clone as 85098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85098-1-RR (ND4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85098-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02094-1

  Sandwich ELISA standard curve of MP02094-1, Human ND4 Recombinant Matched Antibody Pair - PBS only. 85098-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01674. 85098-2-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

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  Sample test of MP02094-1

  The mean ND4 concentration was determined to be 23.9 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

ND4 Uni-rAb^TM Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85098-1-PBS

Clone No.242422E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

MT-ND4, 242422E2, MT ND4, MTND4, NADH4

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Product Information

85098-1-PBS targets ND4 as part of a matched antibody pair:

MP02094-1: 85098-1-PBS capture and 85098-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: NADH dehydrogenase, subunit 4 (complex I)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 43-52 kDa
• GenBank Accession Number: YP_003024035
• Gene Symbol: ND4
• Gene ID (NCBI): 4538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P03905
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.