ND4 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• ND4 Recombinant monoclonal antibody (85098-1-RR)

• 

  WB analysis using 85098-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85098-1-RR (ND4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 85098-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85098-1-RR (ND4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 85098-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85098-1-RR (ND4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ND4 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 85098-1-RR

Clone No.242422E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MT-ND4, 242422E2, MT ND4, MTND4, NADH4

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, SGC-7901 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85098-1-RR targets ND4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: NADH dehydrogenase, subunit 4 (complex I)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 43-52 kDa
• GenBank Accession Number: YP_003024035
• Gene Symbol: ND4
• Gene ID (NCBI): 4538
• RRID: AB_3744010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P03905
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NADH dehydrogenase 4 (ND4) is one of the core subunits of mitochondrial respiratory chain Complex I and is essential for cellular energy production. Complex I is the first step involved in cellular aerobic respiration (i.e., oxidative phosphorylation), responsible for catalyzing the transfer of electrons from NADH to coenzyme Q10 (ubiquinone), thereby initiating the entire electron transport chain.