|Positive WB detected in||C6 cells, HEK-293 cells, HT-1080 cells, A549 cells, COS-7 cells, Jurkat cells|
|Positive IP detected in||C6 cells|
|Positive IHC detected in||human breast cancer tissue, human lung cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21698-1-AP is NEDD4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NEDD4 fusion protein Ag16291|
|Full Name||neural precursor cell expressed, developmentally down-regulated 4|
|Calculated molecular weight||1319 aa, 149 kDa|
|Observed molecular weight||115 kDa|
|GenBank accession number||BC152452|
|Gene ID (NCBI)||4734|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2,NEDL1 AND NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).
Biochem Biophys Res Commun
YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.
J Exp Clin Cancer Res
N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21.
Cell Death Discov
JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.
Human cytomegalovirus UL42 protein inhibits the degradation of glycoprotein B through inhibition of Nedd4 family ubiquitin E3 ligases.
Biochem Biophys Res Commun
O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.
Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tongbin (Verified Customer) (08-25-2020)
This NEDD4 antibody works very well in western blots.