NEDD4 Polyclonal antibody

• Featured Product
• KD/KO Validated

NEDD4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 21698-1-AP

Synonyms

KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1

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Validation Data Gallery

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  WB analysis using 21698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21698-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 21698-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 21698-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21698-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 21698-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of C6 using 21698-1-AP

  IP Result of anti-NEDD4 (IP:21698-1-AP, 4ug; Detection:21698-1-AP 1:500) with C6 cells lysate 1600ug.

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  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: C6 cells, HEK-293 cells, HT-1080 cells, A549 cells, COS-7 cells, Jurkat cells
• Positive IP detected in: C6 cells
• Positive IHC detected in: human breast cancer tissue, human lung cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 21698-1-AP is NEDD4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEDD4 fusion protein Ag16291
• Full Name: neural precursor cell expressed, developmentally down-regulated 4
• Calculated molecular weight: 1319 aa, 149 kDa
• Observed molecular weight: 115 kDa
• GenBank accession number: BC152452
• Gene symbol: NEDD4
• Gene ID (NCBI): 4734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2,NEDL1 AND NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.; Authors - Siming Li
human	WB	Theranostics; PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.; Authors - Xiaoxiao Wang
human	WB	Cell Death Differ; Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer.; Authors - Pengfei Zhang
human	WB, CoIP	Clin Transl Med; Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.; Authors - Jing Chen
human	WB	Diabetes; Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.; Authors - Yuguo Niu
human	IHC	Cell Mol Gastroenterol Hepatol; Distinct and Overlapping Roles of Hippo Effectors YAP and TAZ During Human and Mouse Hepatocarcinogenesis.; Authors - Haichuan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This NEDD4 antibody works very well in western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart