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  • KD/KO Validated

NEDD4 Polyclonal antibody

NEDD4 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, monkey and More (1)





Cat no : 21698-1-AP


KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1

Tested Applications

Positive WB detected inC6 cells, HEK-293 cells, HT-1080 cells, A549 cells, COS-7 cells, Jurkat cells
Positive IP detected inC6 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21698-1-AP is NEDD4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD4 fusion protein Ag16291
Full Name neural precursor cell expressed, developmentally down-regulated 4
Calculated molecular weight 1319 aa, 149 kDa
Observed molecular weight 115 kDa
GenBank accession numberBC152452
Gene symbol NEDD4
Gene ID (NCBI) 4734
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2,NEDL1 AND NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).


Product Specific Protocols
WB protocol for NEDD4 antibody 21698-1-APDownload protocol
IHC protocol for NEDD4 antibody 21698-1-APDownload protocol
IP protocol for NEDD4 antibody 21698-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

YY1-regulated LINC00152 promotes triple negative breast cancer progression by affecting on stability of PTEN protein.

Authors - Xi Shen

J Exp Clin Cancer Res

N-myc downstream-regulated gene 1 inhibits the proliferation of colorectal cancer through emulative antagonizing NEDD4-mediated ubiquitylation of p21.

Authors - Sen Zhang

Cell Death Discov

JAC1 suppresses proliferation of breast cancer through the JWA/p38/SMURF1/HER2 signaling.

Authors - Yanlin Ren
  • KD Validated
humanWB, IF, IP

Microbiol Immunol

Human cytomegalovirus UL42 protein inhibits the degradation of glycoprotein B through inhibition of Nedd4 family ubiquitin E3 ligases.

Authors - Tetsuo Koshizuka

Biochem Biophys Res Commun

O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.

Authors - Kuan Jiang

Life Sci

Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.

Authors - Yanting Zhu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tongbin (Verified Customer) (08-25-2020)

This NEDD4 antibody works very well in western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart