Name: NEDD4 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IP, IHC, ELISA
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C6 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP(NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IP Result of anti-NEDD4 (IP:21698-1-AP, 4ug; Detection:21698-1-AP 1:500) with C6 cells lysate 1600ug.

NEDD4 Antibody IHC human liver tissue 21698-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 21698-1-AP( NEDD4 Antibody) at dilution of 1:50

Species specificity:

human, mouse, rat, monkey

Positive WB detected in:

C6 cells, A549 cells, COS-7 cells, HEK-293 cells, HT-1080 cells

Positive IP detected in:

C6 cells

Positive IHC detected in:

human liver tissue, human breast cancer tissue, human lung cancer tissue

Recommended dilution:

WB : 1:500-1:2400

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

NEDD4 fusion protein Ag16291

Full name:

neural precursor cell expressed, developmentally down-regulated 4

Calculated molecular weight:

1319aa,149 kDa

Observed molecular weight:

115 kDa

GenBank accession number:

BC152452

Gene ID (NCBI):

4734

Gene symbol

NEDD4

Synonyms

KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1

Background

NEDD4-1(Neural precursor cell expressed developmentally down-regulated protein 4) is also named as NEDD4 and KIAA0093. It plays an essential role for the activation of the genetic program involved in the terminal maturation stage of invariant natural killer T-cell development(PMID:21949387). And it is differentially expressed in mammary epithelial cells and plays a role in luminal lineage determination(PMID:20351249). NEDD4-1 is a key regulator of FGFR1 endocytosis and signalling during neuronal differentiation and embryonic development(PMID:21765395). This antibody can recognize NEDD4-1 and NEDD4-2.

Showing 1- [5] of [5] Publications

Author:		Huake Sun
Pubmed ID:		28714370
Journal:		Tumour Biol
Title:		Phosphatase and tensin homolog deleted on chromosome 10 degradation induced by NEDD4 promotes acquired erlotinib resistance in non-small-cell lung cancer.
Application:		WB
KD validated
Species:		human

Author:		Siming Li
Pubmed ID:		28784777
Journal:		Proc Natl Acad Sci U S A
Title:		Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.
Application:		WB

Species:		mouse

Author:		Katrin Juenemann
Pubmed ID:		29362455
Journal:		Sci Rep
Title:		Dynamic recruitment of ubiquitin to mutant huntingtin inclusion bodies.
Application:		WB

Species:		rat

Author:		Kuan Jiang
Pubmed ID:		26876577
Journal:		Biochem Biophys Res Commun
Title:		O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.
Application:		WB

Species:		human

Author:		Yanting Zhu
Pubmed ID:		28212825
Journal:		Life Sci
Title:		Inhibition of ubiquitin proteasome function prevents monocrotaline-induced pulmonary arterial remodeling.
Application:		WB

Species:		rat

WB Figures

A549 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP(NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

C6 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP(NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

NEDD4 Antibody WB COS-7 cells 21698-1-AP

COS-7 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP(NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

NEDD4 Antibody WB HEK-293 cells 21698-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP(NEDD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

NEDD4 Antibody WB HT-1080 cells 21698-1-AP

HT-1080 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP(NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

IHC Figures

NEDD4 Antibody IHC human breast cancer tissue 21698-1-AP

Immunohistochemistry of paraffin-embedded human breast cancer slide using 21698-1-AP( NEDD4 Antibody) at dilution of 1:50

Immunohistochemistry of paraffin-embedded human liver tissue slide using 21698-1-AP( NEDD4 Antibody) at dilution of 1:50

NEDD4 Antibody IHC human lung cancer tissue 21698-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer slide using 21698-1-AP( NEDD4 Antibody) at dilution of 1:50

IP Figures

IP Result of anti-NEDD4 (IP:21698-1-AP, 4ug; Detection:21698-1-AP 1:500) with C6 cells lysate 1600ug.

NEDD4 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 21698-1-AP

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Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background

Showing 1- [5] of [5] Publications

WB Figures

IHC Figures

IP Figures

NEDD4 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 21698-1-AP

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Special offer: Add control/tag antibody for $99

Tested Applications

Protocols

Product-specific protocols

Standard Protocols

Product Information

Immunogen Information

Background