NEDD4L Polyclonal antibody

• Featured Product
• KD/KO Validated

NEDD4L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 13690-1-AP

Synonyms

NEDD4.2, KIAA0439, hNEDD4-2, HECT-type E3 ubiquitin transferase NED4L, EC:2.3.2.26

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Validation Data Gallery

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  WB analysis using 13690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 13690-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 13690-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13690-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13690-1-AP (NEDD4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 13690-1-AP

  IP result of anti-NEDD4L (IP:13690-1-AP, 4ug; Detection:13690-1-AP 1:300) with C6 cells lysate 1000 ug.

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13690-1-AP (NEDD4L Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 13690-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13690-1-AP (NEDD4L antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 13690-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (13690-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, HT-1080 cells, PC-3 cells, PC-12 cells
• Positive IP detected in: C6 cells
• Positive IHC detected in: human prostate cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 33 publications below
• IHC: See 12 publications below
• IF: See 7 publications below
• IP: See 7 publications below
• CoIP: See 5 publications below

Product Information

13690-1-AP targets NEDD4L in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEDD4L fusion protein Ag4565
• Full Name: neural precursor cell expressed, developmentally down-regulated 4-like
• Calculated Molecular Weight: 854 aa, 96 kDa
• Observed Molecular Weight: 112 kDa, 125 kDa
• GenBank Accession Number: BC032597
• Gene Symbol: NEDD4L
• Gene ID (NCBI): 23327
• RRID: AB_2149326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Publications

Species	Application	Title
human	WB,IHC,IF	Nat Genet; Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.; Authors - Loïc Broix
mouse	WB	J Exp Med; USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.; Authors - Siyuan Chen
rat	WB	Cell Death Differ; Aagab acts as a novel regulator of NEDD4-1-mediated Pten nuclear translocation to promote neurological recovery following hypoxic-ischemic brain damage.; Authors - Chunfang Dai
human	WB	Theranostics; JP1 suppresses proliferation and metastasis of melanoma through MEK1/2 mediated NEDD4L-SP1-Integrin αvβ3 signaling.; Authors - Jiahua Cui
rat,human	CoIP,IF	Pharmacol Res; Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2.; Authors - Lifang Huo
mouse	WB, IP, IF	Mol Cell Biol; Plk1 Phosphorylation of PTEN Causes a Tumor-Promoting Metabolic State.; Authors - Zhiguo Li