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NEDD4L Polyclonal antibody

NEDD4L Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13690-1-AP

Synonyms

hNedd4 2, KIAA0439, NEDD4 2, NEDD4.2, NEDD4L, NEDL3, RSP5



Tested Applications

Positive WB detected inC6 cells, HT-1080 cells, PC-3 cells, PC-12 cells
Positive IP detected inC6 cells
Positive IHC detected inhuman prostate cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13690-1-AP targets NEDD4L in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEDD4L fusion protein Ag4565
Full Name neural precursor cell expressed, developmentally down-regulated 4-like
Calculated molecular weight 854 aa, 96 kDa
Observed molecular weight 112 kDa, 125 kDa
GenBank accession numberBC032597
Gene symbol NEDD4L
Gene ID (NCBI) 23327
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.

Protocols

Product Specific Protocols
WB protocol for NEDD4L antibody 13690-1-APDownload protocol
IHC protocol for NEDD4L antibody 13690-1-APDownload protocol
IF protocol for NEDD4L antibody 13690-1-APDownload protocol
IP protocol for NEDD4L antibody 13690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Nat Genet

Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.

Authors - Loïc Broix
mouseWB

J Exp Med

USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.

Authors - Siyuan Chen
ratWB

Cell Death Differ

Aagab acts as a novel regulator of NEDD4-1-mediated Pten nuclear translocation to promote neurological recovery following hypoxic-ischemic brain damage.

Authors - Chunfang Dai
humanWB

Theranostics

JP1 suppresses proliferation and metastasis of melanoma through MEK1/2 mediated NEDD4L-SP1-Integrin αvβ3 signaling.

Authors - Jiahua Cui
rat,humanCoIP,IF

Pharmacol Res

Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2.

Authors - Lifang Huo
mouseWB, IP, IF

Mol Cell Biol

Plk1 Phosphorylation of PTEN Causes a Tumor-Promoting Metabolic State.

Authors - Zhiguo Li