|Positive WB detected in||C6 cells, HT-1080 cells, PC-3 cells, PC-12 cells|
|Positive IP detected in||C6 cells|
|Positive IHC detected in||human prostate cancer tissue, human stomach cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13690-1-AP targets NEDD4L in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NEDD4L fusion protein Ag4565|
|Full Name||neural precursor cell expressed, developmentally down-regulated 4-like|
|Calculated molecular weight||854 aa, 96 kDa|
|Observed molecular weight||112 kDa, 125 kDa|
|GenBank accession number||BC032597|
|Gene ID (NCBI)||23327|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.
|Product Specific Protocols|
|WB protocol for NEDD4L antibody 13690-1-AP||Download protocol|
|IHC protocol for NEDD4L antibody 13690-1-AP||Download protocol|
|IF protocol for NEDD4L antibody 13690-1-AP||Download protocol|
|IP protocol for NEDD4L antibody 13690-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Exp Pathol
Nedd4L modulates the transcription of metalloproteinase-1 and -13 genes to increase the invasive activity of gallbladder cancer.
Int J Exp Pathol
Pathobiological properties of the ubiquitin ligase Nedd4L in melanoma.
Decreased expression of Nedd4L correlates with poor prognosis in gastric cancer patient.
Int J Med Sci
The correlation between NEDD4L and HIF-1α levels as a gastric cancer prognostic marker.
Int J Oncol
Oncogenic function of p34SEI-1 via NEDD4?1?mediated PTEN ubiquitination/degradation and activation of the PI3K/AKT pathway.
Ehrlichia chaffeensis TRP120 moonlights as a HECT E3 ligase involved in self and host ubiquitination to influence protein interactions and stability for intracellular survival.