NF-H/NF200 Polyclonal antibody

NF-H/NF200 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18934-1-AP

Synonyms

200 kDa neurofilament protein, NEFH, NEH, Neurofilament H, NF, NF H, NF200, NF-H



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18934-1-AP targets NF-H/NF200 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-H/NF200 fusion protein Ag13517
Full Name neurofilament, heavy polypeptide
Calculated Molecular Weight 112 kDa
Observed Molecular Weight 200 kDa, 140-160 kDa
GenBank Accession NumberBC014185
Gene Symbol NEFH
Gene ID (NCBI) 4744
RRIDAB_10640801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.

Protocols

Product Specific Protocols
WB protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
IHC protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
IF protocol for NF-H/NF200 antibody 18934-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Metab

Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes

Authors - Junpeng Yang
humanIHC

Small Methods

Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration

Authors - Shunyi Lu

Acta Neuropathol Commun

A robust TDP-43 knock-in mouse model of ALS.

Authors - Shih-Ling Huang
mouseWB

PLoS Genet

Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules.

Authors - Rachid El Fatimy
mouseIHC

Glia

ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.

Authors - Lukasz Mateusz Szewczyk
ratIF

Int J Pharm

Berberine-loaded MSC-derived sEVs encapsulated in injectable GelMA hydrogel for spinal cord injury repair

Authors - Heng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Azita (Verified Customer) (06-02-2021)

Western blotting analysis of NSC34 cells using NF-H Polyclonal Antibody showed strong bonds when the antibody was used at the dilution of 1:400.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: NSC34 cells