Tested Applications
| Positive WB detected in | pig cerebellum tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | rat cerebellum tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF-Fro detected in | rat brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60331-1-Ig targets NF-H/NF200 in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13517 Product name: Recombinant human NF200 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC014185 Sequence: MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARV Predict reactive species |
| Full Name | neurofilament, heavy polypeptide |
| Calculated Molecular Weight | 112 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC014185 |
| Gene Symbol | NF-H |
| Gene ID (NCBI) | 4744 |
| RRID | AB_2881440 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12036 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for NF-H/NF200 antibody 60331-1-Ig | Download protocol |
| IF protocol for NF-H/NF200 antibody 60331-1-Ig | Download protocol |
| IHC protocol for NF-H/NF200 antibody 60331-1-Ig | Download protocol |
| WB protocol for NF-H/NF200 antibody 60331-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NF-H/NF200 Monoclonal antibody (catalog number 60331-1-Ig) has 51 citations. These appear across journals including Nature Communications, Advanced Materials, Advanced Science, Biomaterials, Science Advances, ACS Nano, Journal of Neuroscience, Molecular Neurobiology, and Cell Death & Differentiation. The research fields are predominantly neuroscience and neurobiology, focusing on peripheral and central nerve regeneration, spinal cord injury, neuropathic pain, stroke, neuroinflammation, and neurodegenerative diseases such as Alzheimer's and ALS. Applications are mainly immunofluorescence, Western blot, and IHC.
| Species | Application | Title |
|---|---|---|
Adv Mater Photo-Responsive H2S Composite System Regulates the Nerve Regeneration Microenvironment Through Multiple Pathways | ||
Bioact Mater Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
ACS Nano Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia | ||
Adv Sci (Weinh) Tumor-Associated Sympathetic Nerves Promote the Progression of Epstein-Barr Virus-Positive Diffuse Large B-Cell Lymphoma |
Reviews
What customers say
The single review for this neurofilament antibody reports excellent performance in immunofluorescence on human brain FFPE cortex at a 1:200 dilution. The reviewer rated it 5 stars, noting that it clearly labeled neurons and worked perfectly for their application. Overall, the feedback is highly positive, with no issues or concerns raised.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (07-02-2024) | This neurofilament antibody worked perfectly showing my neurons in human brain FFPE cortex
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