Validation Data Gallery
|Positive WB detected in||pig cerebellum tissue, rat brain tissue, mouse brain tissue|
|Positive IHC detected in||rat cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, rat brain tissue|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:4000-1:16000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60331-1-Ig targets NF-H/NF200 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
|Tested Reactivity||human, pig, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||NF-H/NF200 fusion protein Ag13517|
|Full Name||neurofilament, heavy polypeptide|
|Calculated molecular weight||112 kDa|
|Observed molecular weight||200 kDa|
|GenBank accession number||BC014185|
|Gene ID (NCBI)||4744|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.
|Product Specific Protocols|
|WB protocol for NF-H/NF200 antibody 60331-1-Ig||Download protocol|
|IHC protocol for NF-H/NF200 antibody 60331-1-Ig||Download protocol|
|IF protocol for NF-H/NF200 antibody 60331-1-Ig||Download protocol|
|FC protocol for NF-H/NF200 antibody 60331-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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