NF-H/NF200 Monoclonal antibody

NF-H/NF200 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, pig, mouse, rat







Cat no : 60331-1-Ig


200 kDa neurofilament protein, NEFH, NEH, neurofilament, NF, NF H, NF200, NF-H

Tested Applications

Positive WB detected inpig cerebellum tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, rat brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60331-1-Ig targets NF-H/NF200 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NF-H/NF200 fusion protein Ag13517
Full Name neurofilament, heavy polypeptide
Calculated Molecular Weight 112 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC014185
Gene Symbol NEFH
Gene ID (NCBI) 4744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.


Product Specific Protocols
WB protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
IHC protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
IF protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
FC protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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