NF-H/NF200 Monoclonal antibody

NF-H/NF200 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C7

Cat no : 60331-1-Ig

Synonyms

200 kDa neurofilament protein, NEFH, NEH, neurofilament, NF, NF H, NF200, NF-H



Tested Applications

Positive WB detected inpig cerebellum tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inrat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60331-1-Ig targets NF-H/NF200 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NF-H/NF200 fusion protein Ag13517
Full Name neurofilament, heavy polypeptide
Calculated molecular weight 112 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC014185
Gene symbol NEFH
Gene ID (NCBI) 4744
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.

Protocols

Product Specific Protocols
WB protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
IHC protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
IF protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
FC protocol for NF-H/NF200 antibody 60331-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Chem Neuroanat

Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.

Authors - Huangao Zhou
mouseIHC

Front Cell Neurosci

Ursolic Acid Ameliorates Spinal Cord Injury in Mice by Regulating Gut Microbiota and Metabolic Changes.

Authors - Zi-Jie Rong
ratIF

J Neuroinflammation

Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.

Authors - Qing Liu
ratIF

Biomaterials

Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.

Authors - Xianzhen Dong