NF-H/NF200 Monoclonal antibody, PBS Only

NF-H/NF200 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C7

Cat no : 60331-1-PBS

Synonyms

200 kDa neurofilament protein, NEFH, NEH, neurofilament, NF, NF H, NF200, NF-H

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Validation Data Gallery

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  WB analysis of pig cerebellum using 60331-1-Ig (same clone as 60331-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 60331-1-Ig (same clone as 60331-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 60331-1-Ig (same clone as 60331-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 60331-1-Ig (NF-H antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-H/NF200 antibody (60331-1-Ig, Clone: 1A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 60331-1-Ig (same clone as 60331-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 60331-1-Ig (NF200 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 60331-1-Ig (same clone as 60331-1-PBS)

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-H/NF200 (60331-1-Ig, Clone:1A3C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60331-1-PBS targets NF-H/NF200 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NF-H/NF200 fusion protein Ag13517
• Full Name: neurofilament, heavy polypeptide
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: BC014185
• Gene Symbol: NEFH
• Gene ID (NCBI): 4744
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.