NKp46/NCR1 Polyclonal antibody

• 

  IHC staining of mouse spleen using 31984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31984-1-AP (NKp46/NCR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 31984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31984-1-AP (NKp46/NCR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NKp46/NCR1 Polyclonal Antibody for IHC, ELISA

Cat No. 31984-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, ELISA

Synonyms

Ncr1, Natural killer cell p46-related protein, mNKp46, Lymphocyte antigen 94, Ly94

---

Tested Applications

• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31984-1-AP targets NKp46/NCR1 in IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: natural cytotoxicity triggering receptor 1
• Calculated Molecular Weight: 37kDa
• GenBank Accession Number: NM_010746.3
• Gene Symbol: NKp46/NCR1
• Gene ID (NCBI): 17086
• RRID: AB_3670165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8C567
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).