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  • KD/KO Validated

B23/NPM1 Monoclonal antibody

B23/NPM1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat







Cat no : 60096-1-Ig


B23, B23/NPM1, NPM, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

Tested Applications

Positive WB detected inHSC-T6 cells, HepG2 cells, LNCaP cells, HeLa cells, RAW 264.7 cells, ROS1728 cells, NIH/3T3 cells, C6 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60096-1-Ig targets B23/NPM1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen B23/NPM1 fusion protein Ag7415
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC002398
Gene Symbol NPM1
Gene ID (NCBI) 4869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.


Product Specific Protocols
WB protocol for B23/NPM1 antibody 60096-1-IgDownload protocol
IHC protocol for B23/NPM1 antibody 60096-1-IgDownload protocol
IF protocol for B23/NPM1 antibody 60096-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.

Authors - Pengbo Cao

Nat Commun

A redox mechanism underlying nucleolar stress sensing by nucleophosmin.

Authors - Kai Yang
  • KD Validated

Nat Commun

Menin enhances c-Myc-mediated transcription to promote cancer progression.

Authors - Gongwei Wu

Aging Cell

Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.

Authors - Patricia Gomez-Suaga

Mol Neurodegener

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Authors - Qingyang Zhang

Hum Mol Genet

Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.

Authors - Zhouteng Tao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tatyana (Verified Customer) (12-04-2023)

Was used for nucleolar staining, very strong specific signal

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human / HeLa cells
B23/NPM1 Antibody Immunofluorescence validation (1:1000 dilution) in Human / HeLa cells (Cat no:60096-1-Ig)