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B23/NPM1 Monoclonal antibody
B23/NPM1 Monoclonal Antibody for IF, IHC, WB,ELISA
Cat no : 60096-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, HepG2 cells, C6 cells, ROS1728 cells, NIH/3T3 cells, RAW 264.7 cells, MCF-7 cells, HEK-293 cells, Jurkat cells|
|Positive IHC detected in||human breast cancer tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
60096-1-Ig targets B23/NPM1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||B23/NPM1 fusion protein Ag7415|
|Full Name||nucleophosmin (nucleolar phosphoprotein B23, numatrin)|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||35-38 kDa|
|GenBank accession number||BC002398|
|Gene ID (NCBI)||4869|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.
Myc-mediated SDHA acetylation triggers epigenetic regulation of gene expression and tumorigenesis.
Cell Death Dis
HO-1 nuclear accumulation and interaction with NPM1 protect against stress-induced endothelial senescence independent of its enzymatic activity.
Acta Biochim Biophys Sin (Shanghai)
Assessment of SENP3-interacting proteins in hepatocytes treated with diethylnitrosamine by BioID assay.
Disruption of ER-mitochondria tethering and signalling in C9orf72-associated amyotrophic lateral sclerosis and frontotemporal dementia.
Identification of the key genes and pathways in prostate cancer.