NPM3 Recombinant antibody

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  WB analysis using 85156-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85156-4-RR (NPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

NPM3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85156-4-RR

Clone No.242863C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TMEM123, PORMIN, Nucleoplasmin-3, Nucleoplasmin 3, nucleophosmin/nucleoplasmin, 3

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Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells, K-562 cells, Raji cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85156-4-RR targets NPM3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: NPM3 fusion protein Ag2564
• Full Name: nucleophosmin/nucleoplasmin, 3
• Calculated Molecular Weight: 178 aa, 19 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC054868
• Gene Symbol: NPM3
• Gene ID (NCBI): 10360
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75607
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NPM3, a member of the nucleophosmin/ nucleoplasmin family, lacks intrinsic histone chaperone activity, inhibits histone assembly activity of NPM1 in vitro, and dramatically enhances transcription in a cellular system(PMID: 20073534). Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. The NPM3 protein migrated in SDS-PAGE gels as a doublet with apparent molecular masses of 25 and 27 kDa, which are larger than the 20.5 kDa mass predicted by the deduced amino acid sequence including the HA tag (PMID: 11722795). The NPM3 antibody can detect two bands with MW between 20-27 kDa.