|Positive WB detected in||HepG2 cells, HSC-T6 cells, 4T1 cells, L02 cells, K-562 cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Sample-dependent, check data in validation data gallery|
67240-1-Ig targets NQO1 in WB, IHC,ELISA applications and shows reactivity with Human, Rat, mouse samples.
|Tested Reactivity||Human, Rat, mouse|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||NQO1 fusion protein Ag28933|
|Full Name||NAD(P)H dehydrogenase, quinone 1|
|Calculated molecular weight||274 aa, 31 kDa|
|Observed molecular weight||29-31 kDa|
|GenBank accession number||BC007659|
|Gene ID (NCBI)||1728|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )
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