NQO1 Monoclonal antibody
NQO1 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Rat, pig and More (3)
Applications
WB, IHC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1E5G7
Cat no : 67240-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HSC-T6 cells, L02 cells, K-562 cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 59 publications below |
IHC | See 2 publications below |
Product Information
67240-1-Ig targets NQO1 in WB, IHC, FC, ELISA applications and shows reactivity with Human, Rat, pig samples.
Tested Reactivity | Human, Rat, pig |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | NQO1 fusion protein Ag28933 |
Full Name | NAD(P)H dehydrogenase, quinone 1 |
Calculated molecular weight | 274 aa, 31 kDa |
Observed molecular weight | 29-31 kDa |
GenBank accession number | BC007659 |
Gene symbol | NQO1 |
Gene ID (NCBI) | 1728 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )
Protocols
Product Specific Protocols | |
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WB protocol for NQO1 antibody 67240-1-Ig | Download protocol |
IHC protocol for NQO1 antibody 67240-1-Ig | Download protocol |
FC protocol for NQO1 antibody 67240-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue | ||
Int J Mol Sci Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses | ||
Stem Cell Res Ther Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4 | ||
Oxid Med Cell Longev Ivabradine Ameliorates Cardiac Function in Heart Failure with Preserved and Reduced Ejection Fraction via Upregulation of miR-133a | ||
Oxid Med Cell Longev MCC950, the NLRP3 Inhibitor, Protects against Cartilage Degradation in a Mouse Model of Osteoarthritis | ||
Antioxidants (Basel) Dihydromyricetin Enhances Intestinal Antioxidant Capacity of Growing-Finishing Pigs by Activating ERK/Nrf2/HO-1 Signaling Pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hala (Verified Customer) (07-28-2021) | incubation 1.5 hours at room temperature dilution 1/7000 could use more diluted concentration
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