NQO1 Monoclonal antibody

NQO1 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, pig and More (3)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

61

CloneNo.

1E5G7

Reviews (1)

Cat no : 67240-1-Ig

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1

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Validation Data Gallery

• 

  WB analysis using 67240-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67240-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67240-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67240-1-Ig (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 67240-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67240-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67240-1-Ig (NQO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MCF-7 using 67240-1-Ig

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human NQO1 (67240-1-Ig, Clone:1E5G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, HSC-T6 cells, L02 cells, K-562 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 59 publications below
• IHC: See 2 publications below

Product Information

67240-1-Ig targets NQO1 in WB, IHC, FC, ELISA applications and shows reactivity with Human, Rat, pig samples.

• Tested Reactivity: Human, Rat, pig
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NQO1 fusion protein Ag28933
• Full Name: NAD(P)H dehydrogenase, quinone 1
• Calculated molecular weight: 274 aa, 31 kDa
• Observed molecular weight: 29-31 kDa
• GenBank accession number: BC007659
• Gene symbol: NQO1
• Gene ID (NCBI): 1728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )

Publications

Species	Application	Title
rat	WB	Oxid Med Cell Longev; Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue; Authors - Guo-Jie Han
mouse	WB	Int J Mol Sci; Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses; Authors - Jin Sun
human,rat	WB	Stem Cell Res Ther; Mesenchymal stem cells-derived small extracellular vesicles alleviate diabetic retinopathy by delivering NEDD4; Authors - Fengtian Sun
human	WB	Oxid Med Cell Longev; Ivabradine Ameliorates Cardiac Function in Heart Failure with Preserved and Reduced Ejection Fraction via Upregulation of miR-133a; Authors - Jinliang Liu
mouse	WB	Oxid Med Cell Longev; MCC950, the NLRP3 Inhibitor, Protects against Cartilage Degradation in a Mouse Model of Osteoarthritis; Authors - Bowei Ni
pig	WB	Antioxidants (Basel); Dihydromyricetin Enhances Intestinal Antioxidant Capacity of Growing-Finishing Pigs by Activating ERK/Nrf2/HO-1 Signaling Pathway.; Authors - Chuan Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hala (Verified Customer) (07-28-2021)

incubation 1.5 hours at room temperature dilution 1/7000 could use more diluted concentration

• Applications: Western Blot
• Primary Antibody Dilution: 1/7000
• Cell Tissue Type: HepG2

NQO1 Antibody Western Blot validation (1/7000 dilution) in HepG2 (Cat no:67240-1-Ig)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C