NQO1 Monoclonal antibody

NQO1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a


Human, Rat and More (4)







Cat no : 67240-1-Ig


Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1

Tested Applications

Positive WB detected inHepG2 cells, HSC-T6 cells, L02 cells, K-562 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67240-1-Ig targets NQO1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NQO1 fusion protein Ag28933
Full Name NAD(P)H dehydrogenase, quinone 1
Calculated Molecular Weight 274 aa, 31 kDa
Observed Molecular Weight 29-31 kDa
GenBank Accession NumberBC007659
Gene Symbol NQO1
Gene ID (NCBI) 1728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )


Product Specific Protocols
WB protocol for NQO1 antibody 67240-1-IgDownload protocol
IHC protocol for NQO1 antibody 67240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Redox Biol

Remote ischemic conditioning attenuates oxidative stress and inflammation via the Nrf2/HO-1 pathway in MCAO mice

Authors - Ying-Ying Sun

Oxid Med Cell Longev

Xuesaitong Combined with Dexmedetomidine Improves Cerebral Ischemia-Reperfusion Injury in Rats by Activating Keap1/Nrf2 Signaling and Mitophagy in Hippocampal Tissue

Authors - Guo-Jie Han

Oxid Med Cell Longev

Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.

Authors - Ning Ma

Oxid Med Cell Longev

Limonin Inhibits IL-1 β -Induced Inflammation and Catabolism in Chondrocytes and Ameliorates Osteoarthritis by Activating Nrf2

Authors - Jie Jin

Acta Pharmacol Sin

CZ-7, a new derivative of Claulansine F, ameliorates 2VO-induced vascular dementia in rats through a Nrf2-mediated antioxidant responses.

Authors - Dan-Dan Liu

Acta Pharmacol Sin

Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.

Authors - Jia-Wei Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hala (Verified Customer) (07-28-2021)

incubation 1.5 hours at room temperature dilution 1/7000 could use more diluted concentration

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/7000
  • Cell Tissue Type: HepG2
NQO1 Antibody Western Blot validation (1/7000 dilution) in HepG2 (Cat no:67240-1-Ig)