|Positive WB detected in||HepG2 cells, HSC-T6 cells, 4T1 cells, L02 cells, K-562 cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Sample-dependent, check data in validation data gallery|
67240-1-Ig targets NQO1 in WB, IHC,ELISA applications and shows reactivity with Human, Rat, mouse, pig samples.
|Tested Reactivity||Human, Rat, mouse, pig|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||NQO1 fusion protein Ag28933|
|Full Name||NAD(P)H dehydrogenase, quinone 1|
|Calculated molecular weight||274 aa, 31 kDa|
|Observed molecular weight||29-31 kDa|
|GenBank accession number||BC007659|
|Gene ID (NCBI)||1728|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )
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CZ-7, a new derivative of Claulansine F, ameliorates 2VO-induced vascular dementia in rats through a Nrf2-mediated antioxidant responses.
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Food Sci Nutr
Limonin induces apoptosis of HL-60 cells by inhibiting NQO1 activity.
Role of CYP4F2 as a novel biomarker regulating malignant phenotypes of liver cancer cells via the Nrf2 signaling axis.
miR-98-5p Protects Against Cerebral ischemia/reperfusion Injury Through Anti-apoptosis and Anti-oxidative stress in Mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hala (Verified Customer) (07-28-2021)
incubation 1.5 hours at room temperature dilution 1/7000 could use more diluted concentration