NQO1 Monoclonal antibody

NQO1 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, mouse and More (3)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E5G7

Cat no : 67240-1-Ig

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1



Tested Applications

Positive WB detected inHepG2 cells, HSC-T6 cells, 4T1 cells, L02 cells, K-562 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 12 publications below

Product Information

67240-1-Ig targets NQO1 in WB, IHC,ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Cited Reactivity human, mouse, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NQO1 fusion protein Ag28933
Full Name NAD(P)H dehydrogenase, quinone 1
Calculated molecular weight 274 aa, 31 kDa
Observed molecular weight 29-31 kDa
GenBank accession numberBC007659
Gene symbol NQO1
Gene ID (NCBI) 1728
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )

Protocols

Product Specific Protocols
WB protocol for NQO1 antibody 67240-1-IgDownload protocol
IHC protocol for NQO1 antibody 67240-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.

Authors - Ning Ma
ratWB

Acta Pharmacol Sin

CZ-7, a new derivative of Claulansine F, ameliorates 2VO-induced vascular dementia in rats through a Nrf2-mediated antioxidant responses.

Authors - Dan-Dan Liu
humanWB

Acta Pharmacol Sin

Methylene blue prevents osteoarthritis progression and relieves pain in rats via upregulation of Nrf2/PRDX1.

Authors - Jia-Wei Li
humanWB

Food Sci Nutr

Limonin induces apoptosis of HL-60 cells by inhibiting NQO1 activity.

Authors - Yunyi Chen
humanWB

Oncol Lett

Role of CYP4F2 as a novel biomarker regulating malignant phenotypes of liver cancer cells via the Nrf2 signaling axis.

Authors - Shunyuan Wan
humanWB

J Biochem

miR-98-5p Protects Against Cerebral ischemia/reperfusion Injury Through Anti-apoptosis and Anti-oxidative stress in Mice.

Authors - Shan Yu