CoraLite®488-conjugated NQO1 Monoclonal antibody

NQO1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1E5G7

Cat no : CL488-67240

Synonyms

Azoreductase, DHQU, DIA4, DT diaphorase, DTD, Menadione reductase, NQO1, Phylloquinone reductase, QR1, Quinone reductase 1



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67240-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67240 targets NQO1 in IF applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NQO1 fusion protein Ag28933
Full Name NAD(P)H dehydrogenase, quinone 1
Calculated molecular weight 274 aa, 31 kDa
Observed molecular weight 29-31 kDa
GenBank accession numberBC007659
Gene symbol NQO1
Gene ID (NCBI) 1728
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640; 18848868 )

Protocols

Product Specific Protocols
IF protocol for CL488 NQO1 antibody CL488-67240Download protocol
Standard Protocols
Click here to view our Standard Protocols