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  • KD/KO Validated

NR1H2 Monoclonal antibody

NR1H2 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E12B10

Cat no : 60345-1-Ig

Synonyms

Liver X receptor beta, LXR b, LXRB, NER, NER I, NR1H2, Nuclear receptor NER, Oxysterols receptor LXR beta, RIP15, UNR



Tested Applications

Positive WB detected inhuman liver tissue, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

60345-1-Ig targets NR1H2 in WB, IHC,ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NR1H2 fusion protein Ag5635
Full Name nuclear receptor subfamily 1, group H, member 2
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberBC047750
Gene symbol NR1H2
Gene ID (NCBI) 7376
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NR1H2, also known as LXRB, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It preferentially bind to double-stranded oligonucleotide direct repeats, which have the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). It involves in the regulation of cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

Protocols

Product Specific Protocols
WB protocol for NR1H2 antibody 60345-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.

Authors - Chuanrui Ma
humanWB

Biochem Biophys Res Commun

Upregulation of 24(R/S),25-epoxycholesterol and 27-hydroxycholesterol suppresses the proliferation and migration of gastric cancer cells.

Authors - Fenghua Guo
mouseWB

Biochem Biophys Res Commun

Thioredoxin-1 promotes macrophage reverse cholesterol transport and protects liver from steatosis.

Authors - Xiong Wang
humanWB, IHC

Food Funct

Polysaccharide MCP extracted from Morchella esculenta reduces atherosclerosis in LDLR-deficient mice.

Authors - Dandan Wang
mouse,humanWB

J Agric Food Chem

Diosmetin Ameliorates Nonalcoholic Steatohepatitis through Modulating Lipogenesis and Inflammatory Response in a STAT1/CXCL10-Dependent Manner.

Authors - Nanxuan Luo
mouse,humanWB

J Biol Chem

TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells.

Authors - Dan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zehua (Verified Customer) (11-07-2019)

This antibody works well in liver lysates from wild-type and hLXR knockin mouse.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse liver
NR1H2 Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (Cat no:60345-1-Ig)