NR1H2 Monoclonal antibody

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  WB analysis of human liver using 60345-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60345-1-Ig (NR1H2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 60345-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60345-1-Ig (NR1H2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

NR1H2 Monoclonal Antibody for WB, ELISA

Cat No. 60345-1-Ig

Clone No.1E12B10

Publications(12)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IHC, ELISA

Synonyms

1E12B10, Liver X receptor beta, LXR b, LXRB, NER

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Tested Applications

• Positive WB detected in: human liver tissue, pig liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 1 publications below

Product Information

60345-1-Ig targets NR1H2 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5635

  Product name: Recombinant human NR1H2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-461 aa of BC047750

  Sequence: MSSPTTSSLDTPLPGNGPPQPGAPSSSPTVKEEGPEPWPGGPDPDVPGTDEASSACSTDWVIPDPEEEPERKRKKGPAPKMLGHELCRVCGDKASGFHYNVLSCEGCKGFFRRSVVRGGARRYACRGGGTCQMDAFMRRKCQQCRLRKCKEAGMREQCVLSEEQIRKKKIRKQQQQESQSQSQSPVGPQGSSSSASGPGASPGGSEAGSQGSGEGEGVQLTAAQELMIQQLVAAQLQCNKRSFSDQPKVTPWPLGADPQSRDARQQRFAHFTELAIISVQEIVDFAKQVPGFLQLGREDQIALLKASTIEIMLLETARRYNHETECITFLKDFTYSKDDFHRAGLQVEFINPIFEFSRAMRRLGLDDAEYALLIAINIFSADRPNVQEPGRVEALQQPYVEALLSYTRIKRPQDQLRFPRMLMKLVSLRTLSSVHSEQVFALRLQDKKLPPLLSEIWDVHE

• Full Name: nuclear receptor subfamily 1, group H, member 2
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC047750
• Gene Symbol: NR1H2
• Gene ID (NCBI): 7376
• RRID: AB_2881454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P55055
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR1H2, also known as LXRB, is a liver X receptors, which form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. It preferentially bind to double-stranded oligonucleotide direct repeats, which have the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). It involves in the regulation of cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
human,mouse		Acta Pharmacol Sin; Liver X receptor agonists exert antitumor effects against hepatocellular carcinoma via inducing REPS2 expression; Authors - Xiao-Yu He
human	WB	Front Oncol; EGFR/FOXO3A/LXR-α Axis Promotes Prostate Cancer Proliferation and Metastasis and Dual-Targeting LXR-α/EGFR Shows Synthetic Lethality.; Authors - Tingting Chen
human	WB	J Lipid Res; 25-Hydroxycholesterol activates the expression of cholesterol 25-hydroxylase in an LXR-dependent mechanism.; Authors - Ying Liu ; KD Validated
human	WB	J Lipid Res; Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.; Authors - Ying Liu ; KO Validated
mouse,human	WB	J Biol Chem; TL1A inhibits atherosclerosis in apoE-deficient mice by regulating the phenotype of vascular smooth muscle cells.; Authors - Dan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zehua (Verified Customer) (11-07-2019)

This antibody works well in liver lysates from wild-type and hLXR knockin mouse.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver

NR1H2 Antibody Western Blot, validation (1:1000 dilution) in Mouse liver (Cat no:60345-1-Ig)