|Positive WB detected in||HEK-293 cells, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human colon tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21854-1-AP is NR3C2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NR3C2 fusion protein Ag16410|
|Full Name||nuclear receptor subfamily 3, group C, member 2|
|Calculated molecular weight||984 aa, 107 kDa|
|Observed molecular weight||94-100 kDa|
|GenBank accession number||BC111758|
|Gene ID (NCBI)||4306|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.This antibody is a rabbit polyclonal antibody, sepcifically reacts with the 107kd MCR protein.
|Product Specific Protocols|
|WB protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IHC protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IF protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IP protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Identification of mineralocorticoid receptor target genes in the mouse hippocampus.
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Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Hypertension by Modulating the Hsa-miR-124-3p/Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2) and Hsa-miR-135a-5p/NR3C2 Axis.
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Aldosterone from endometrial glands is benefit for human decidualization.
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Huoxue Jiedu Huayu Formula Alleviates Cell Pyroptosis in Contralateral Kidneys of 6-Month-Old UUO Rats through the NLRP3/Caspase-1/IL-1β Pathway.
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Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro.
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Age‑related changes in mineralocorticoid receptors in rat hearts.