Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, HEK-293 cells, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
21854-1-AP targets NR3C2 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NR3C2 fusion protein Ag16410|
|Full Name||nuclear receptor subfamily 3, group C, member 2|
|Calculated molecular weight||984 aa, 107 kDa|
|Observed molecular weight||94-110 kDa|
|GenBank accession number||BC111758|
|Gene ID (NCBI)||4306|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.
|Product Specific Protocols|
|WB protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IHC protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IF protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IP protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Paloma (Verified Customer) (06-01-2022)
Good performance in ChIP