|Positive WB detected in||HEK-293 cells, HeLa cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human kidney tissue, human colon tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21854-1-AP is NR3C2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NR3C2 fusion protein Ag16410|
|Full Name||nuclear receptor subfamily 3, group C, member 2|
|Calculated molecular weight||984 aa, 107 kDa|
|Observed molecular weight||94-100 kDa|
|GenBank accession number||BC111758|
|Gene ID (NCBI)||4306|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.This antibody is a rabbit polyclonal antibody, sepcifically reacts with the 107kd MCR protein.
|Product Specific Protocols|
|WB protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IHC protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IF protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|IP protocol for NR3C2 antibody 21854-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Identification of mineralocorticoid receptor target genes in the mouse hippocampus.
Med Sci Monit
Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Hypertension by Modulating the Hsa-miR-124-3p/Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2) and Hsa-miR-135a-5p/NR3C2 Axis.
Cell Death Dis
Aldosterone from endometrial glands is benefit for human decidualization.
Med Sci Monit
Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro.
Mol Med Rep
Age‑related changes in mineralocorticoid receptors in rat hearts.
Response of the maternal hypothalamus to cold stress during late pregnancy in rats.