NR3C2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NR3C2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

27

Reviews (1)

Cat no : 21854-1-AP

Synonyms

MR, MLR, MCR

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Validation Data Gallery

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  WB analysis of mouse kidney using 21854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21854-1-AP

  WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells.

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  WB analysis using 21854-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21854-1-AP

  IP result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human kidney using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21854-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HEK-293 using 21854-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 10 publications below
• IF: See 7 publications below
• ChIP: See 3 publications below

Product Information

21854-1-AP targets NR3C2 in WB, IHC, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NR3C2 fusion protein Ag16410
• Full Name: nuclear receptor subfamily 3, group C, member 2
• Calculated Molecular Weight: 984 aa, 107 kDa
• Observed Molecular Weight: 94-110 kDa
• GenBank Accession Number: BC111758
• Gene Symbol: NR3C2
• Gene ID (NCBI): 4306
• RRID: AB_10858785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Publications

Species	Application	Title
rat	IHC	J Pineal Res; Melatonin prevents experimental central serous chorioretinopathy in rats.; Authors - Shanshan Yu
human	WB	EMBO Rep; High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.; Authors - Yukang Yuan
rat	WB	Oncotarget; Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.; Authors - Shuai Lian
rat	IHC,IF	Int Immunopharmacol; UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.; Authors - Fan Yang
rat	WB	Behav Brain Res; Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.; Authors - Shuai Lian
rat	WB	Behav Brain Res; Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.; Authors - Shuai Lian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paloma (Verified Customer) (06-01-2022)

Good performance in ChIP

• Applications: ChIP
• Cell Tissue Type: Immortalized keratinocytes