NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 21854-1-AP

Synonyms

MCR, Mineralocorticoid receptor, MLR, MR, NR3C2



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human colon tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21854-1-AP is NR3C2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR3C2 fusion protein Ag16410
Full Name nuclear receptor subfamily 3, group C, member 2
Calculated molecular weight 984 aa, 107 kDa
Observed molecular weight 94-100 kDa
GenBank accession numberBC111758
Gene symbol NR3C2
Gene ID (NCBI) 4306
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.This antibody is a rabbit polyclonal antibody, sepcifically reacts with the 107kd MCR protein.

Protocols

Product Specific Protocols
WB protocol for NR3C2 antibody 21854-1-APDownload protocol
IHC protocol for NR3C2 antibody 21854-1-APDownload protocol
IF protocol for NR3C2 antibody 21854-1-APDownload protocol
IP protocol for NR3C2 antibody 21854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mousechIP

J Neuroendocrinol

Identification of mineralocorticoid receptor target genes in the mouse hippocampus.

Authors - Lisa T C M van Weert
humanWB

Med Sci Monit

Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Hypertension by Modulating the Hsa-miR-124-3p/Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2) and Hsa-miR-135a-5p/NR3C2 Axis.

Authors - Liju Luo
humanWB,IHC,IF

Cell Death Dis

Aldosterone from endometrial glands is benefit for human decidualization.

Authors - Shu-Yun Li
ratWB

Med Sci Monit

Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro.

Authors - Yu Yang
ratWB,IHC

Mol Med Rep

Age‑related changes in mineralocorticoid receptors in rat hearts.

Authors - Danli Hu
ratWB

Brain Res

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Authors - Di Wang