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  • KD/KO Validated

NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 21854-1-AP

Synonyms

MCR, Mineralocorticoid receptor, MLR, MR, NR3C2



Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

21854-1-AP targets NR3C2 in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR3C2 fusion protein Ag16410
Full Name nuclear receptor subfamily 3, group C, member 2
Calculated molecular weight 984 aa, 107 kDa
Observed molecular weight 94-110 kDa
GenBank accession numberBC111758
Gene symbol NR3C2
Gene ID (NCBI) 4306
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Protocols

Product Specific Protocols
WB protocol for NR3C2 antibody 21854-1-APDownload protocol
IHC protocol for NR3C2 antibody 21854-1-APDownload protocol
IF protocol for NR3C2 antibody 21854-1-APDownload protocol
IP protocol for NR3C2 antibody 21854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

J Pineal Res

Melatonin prevents experimental central serous chorioretinopathy in rats.

Authors - Shanshan Yu
humanWB

EMBO Rep

High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.

Authors - Yukang Yuan
ratWB

Oncotarget

Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.

Authors - Shuai Lian
ratIHC,IF

Int Immunopharmacol

UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.

Authors - Fan Yang
ratWB

Behav Brain Res

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Authors - Shuai Lian
ratWB

Behav Brain Res

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Authors - Shuai Lian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paloma (Verified Customer) (06-01-2022)

Good performance in ChIP

  • Applications: ChIP
  • Cell Tissue Type: Immortalized keratinocytes