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NR3C2 Polyclonal antibody

NR3C2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 21854-1-AP

Synonyms

MCR, Mineralocorticoid receptor, MLR, MR, NR3C2



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21854-1-AP is NR3C2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NR3C2 fusion protein Ag16410
Full Name nuclear receptor subfamily 3, group C, member 2
Calculated molecular weight 984 aa, 107 kDa
Observed molecular weight 94-110 kDa
GenBank accession numberBC111758
Gene symbol NR3C2
Gene ID (NCBI) 4306
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Protocols

Product Specific Protocols
WB protocol for NR3C2 antibody 21854-1-APDownload protocol
IHC protocol for NR3C2 antibody 21854-1-APDownload protocol
IF protocol for NR3C2 antibody 21854-1-APDownload protocol
IP protocol for NR3C2 antibody 21854-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mousechIP

J Neuroendocrinol

Identification of mineralocorticoid receptor target genes in the mouse hippocampus.

Authors - Lisa T C M van Weert
humanWB

Med Sci Monit

Long Non-Coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Promotes Hypertension by Modulating the Hsa-miR-124-3p/Nuclear Receptor Subfamily 3, Group C, Member 2 (NR3C2) and Hsa-miR-135a-5p/NR3C2 Axis.

Authors - Liju Luo
humanWB,IHC,IF

Cell Death Dis

Aldosterone from endometrial glands is benefit for human decidualization.

Authors - Shu-Yun Li
ratIHC

Evid Based Complement Alternat Med

Huoxue Jiedu Huayu Formula Alleviates Cell Pyroptosis in Contralateral Kidneys of 6-Month-Old UUO Rats through the NLRP3/Caspase-1/IL-1β Pathway.

Authors - Xuelian Ma
ratWB

Med Sci Monit

Inhibitory Effects of Oxymatrine on Transdifferentiation of Neonatal Rat Cardiac Fibroblasts to Myofibroblasts Induced by Aldosterone via Keap1/Nrf2 Signaling Pathways In Vitro.

Authors - Yu Yang
ratWB,IHC

Mol Med Rep

Age‑related changes in mineralocorticoid receptors in rat hearts.

Authors - Danli Hu