GCNF Polyclonal antibody

• Featured Product
• KD/KO Validated

GCNF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12712-1-AP

Synonyms

GCNF, GCNF1, Germ cell nuclear factor, hGCNF, hRTR, NR61, NR6A1, RTR

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Validation Data Gallery

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  WB analysis of PC-3 using 12712-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12712-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12712-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12712-1-AP

  IP result of anti-GCNF (IP:12712-1-AP, 4ug; Detection:12712-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 12712-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12712-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: PC-3 cells, K-562 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12712-1-AP targets GCNF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCNF fusion protein Ag3395
• Full Name: nuclear receptor subfamily 6, group A, member 1
• Calculated Molecular Weight: 475 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC030600
• Gene Symbol: GCNF
• Gene ID (NCBI): 2649
• RRID: AB_10640439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein.

Publications

Species	Application	Title
mouse	WB	Mol Neurobiol; Mitofusin 2 Mediates the Protective Effect of NR6A1 Silencing Against Neuronal Injury in Experimental Stroke Models; Authors - Bingtian Xu
mouse	WB,IF	Neuropharmacology; Hippocampal NR6A1 impairs CREB-BDNF signaling and leads to the development of depression-like behaviors in mice.; Authors - Pingping Tan ; KD Validated
mouse	IHC	Cancer Med; microRNA-196a-5p inhibits testicular germ cell tumor progression via NR6A1/E-cadherin axis.; Authors - Xiaowen Liu ; KD Validated
human	WB	J Clin Transl Hepatol; Establishment of a Prognostic Model for Hepatocellular Carcinoma Based on Bioinformatics and the Role of NR6A1 in the Progression of HCC; Authors - Zhong-Hua Lin