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WB analysis using 18296-1-AP
Recombinant fusion protein Ag27444 (KRAS-2A), Ag21645(KRAS-2B), Ag29746 (NRAS), and Ag23265(HRAS) were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 18296-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human malignant melanoma using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human malignant melanoma using 18296-1-AP
Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18296-1-AP (NRAS-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NRAS-Specific Polyclonal antibody

NRAS-Specific Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18296-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HRAS1, NRAS, EC:3.6.5.2, GTPase NRas, N ras

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, Recombinant protein, Jurkat cells, MCF-7 cells
Positive IHC detected in	human colon tissue, human kidney tissue, human colon cancer tissue, rat colon tissue, mouse colon tissue, human malignant melanoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

18296-1-AP targets NRAS-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	neuroblastoma RAS viral (v-ras) oncogene homolog
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC005219
Gene Symbol	NRAS
Gene ID (NCBI)	4893
RRID	AB_2918061
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P01111
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML). The antibody is specific to NRAS, can't recognize HRAS and KRAS.