NRBF2 Polyclonal antibody, PBS Only

• 

  WB analysis using 24858-1-AP (same clone as 24858-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24858-1-AP (NRBF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 24858-1-AP (same clone as 24858-1-PBS)

  IP result of anti-NRBF2 (IP:24858-1-AP, 4ug; Detection:24858-1-AP 1:2000) with HEK-293 cells lysate 2640ug. This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24858-1-AP (same clone as 24858-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24858-1-AP (NRBF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 24858-1-AP (same clone as 24858-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24858-1-AP (NRBF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 24858-1-AP (same clone as 24858-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NRBF2 antibody (24858-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24858-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

NRBF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 24858-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Nuclear receptor-binding factor 2, NRBF-2, NRBF 2, COPR2, COPR1

---

Product Information

24858-1-PBS targets NRBF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NRBF2 fusion protein Ag21403
• Full Name: nuclear receptor binding factor 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 35-37 kDa
• GenBank Accession Number: BC001345
• Gene Symbol: NRBF2
• Gene ID (NCBI): 29982
• RRID: AB_2879759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96F24
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NRBF2 also termed as Nuclear receptor-binding factor 2or COPR is a 287 amino acid protein, which Interacts with PPARA, PPARD and PPARG. NRBF2 as transcription activator may modulate transcriptional activation by target nuclear receptors. NRBF2 is expressed in keratinocytes, liver and placenta. NRBF2, which exists as two isoforms due to alternative splicing, is localized to both the nucleus and the cytoplasm.