NRG1, isoform SMDF Polyclonal antibody

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  WB analysis using 10527-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10527-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10527-1-AP (NRG1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10527-1-AP (NRG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 1x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 10527-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 10527-1-AP (NRG1, isoform SMDF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10527-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10527-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 10527-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NRG1, isoform SMDF antibody (10527-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NRG1, isoform SMDF Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 10527-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

NRG1, NRG1A, ARIA, GGF, GGF2

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Tested Applications

• Positive WB detected in: MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse stomach tissue, human stomach tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 6 publications below

Product Information

10527-1-AP targets NRG1, isoform SMDF in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0803

  Product name: Recombinant human NRG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-225 aa of BC007675

  Sequence: MEIYSPDMSEVAAERSSSPSTQLSADPSLDGLPAAEDMPEPQTEDGRTPGLVGLAVPCCACLEAERLRGCLNSEKICIVPILACLVSLCLCIAGLKWVFVDKIFEYDSPTHLDPGGLGQDPIISLDATAASALWVSSEAYTSPVSRAQSESEVQVTVQGDKAVVSFEPSAAPTPKNRIFAFSFLPSTAPSFPSPTRNPEVRTPKSATQPQTTETNLQTAPKLSTS

• Full Name: neuregulin 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007675
• Gene Symbol: NRG1
• Gene ID (NCBI): 3084
• RRID: AB_2154659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02297
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuregulin 1 (NRG1) is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. NRG1 and its receptor ErbB tyrosine kinase are expressed not only in the developing nervous system, but also in the adult brain. Studies has been reportrd, the full-length type III NRG1 (NRG1 isoform SMDF) is more than 110 kDa, the 70-75 kDa band is a membrane bound form and it can be cleaved by an unknown enzyme releases the ∼45 kDa secreted form of NRG1(PMID: 21047912, PMID: 11425901). The immunogen of this antibody is against NRG1 isoform SMDF.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	ACS Biomater Sci Eng; Sericin Nerve Guidance Conduit Delivering Therapeutically Repurposed Clobetasol for Functional and Structural Regeneration of Transected Peripheral Nerves; Authors - Lei Zhang
mouse	WB,IHC,IF	Ecotoxicol Environ Saf; Prenatal di-(2-ethylhexyl) phthalate exposure induced myocardial cytotoxicity via the regulation of the NRG1-dependent ErbB2/ErbB4-PI3K/AKT signaling pathway in fetal mice.; Authors - Dongmin Yu
human	IHC	J Exp Clin Cancer Res; miR-296-5p suppresses EMT of hepatocellular carcinoma via attenuating NRG1/ERBB2/ERBB3 signaling.; Authors - Dong-Min Shi
mouse	WB	Am J Physiol Renal Physiol; ErbB4 deletion accelerates renal fibrosis following renal injury.; Authors - Fenghua Zeng
Human	WB,IHC	Int J Oncol; NRG1 regulates redox homeostasis via NRF2 in papillary thyroid cancer.; Authors - Ting-Ting Zhang
human	IF	Folia Neuropathol; miR-143-3p inhibition promotes neuronal survival in an Alzheimer's disease cell model by targeting neuregulin-1.; Authors - Chengyan Sun