NSBP1 Polyclonal antibody, PBS Only

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  WB analysis of PC-3 using 23955-1-AP (same clone as 23955-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23955-1-AP (same clone as 23955-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 23955-1-AP (same clone as 23955-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23955-1-AP (same clone as 23955-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 23955-1-AP (same clone as 23955-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSBP1 antibody (23955-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23955-1-PBS in a different storage buffer formulation.

NSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 23955-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Nucleosome-binding protein 1, NSBP 1, HMGN5, High mobility group nucleosome-binding domain-containing protein 5

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Product Information

23955-1-PBS targets NSBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSBP1 fusion protein Ag20884
• Full Name: nucleosomal binding protein 1
• Calculated Molecular Weight: 282 aa, 32 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005342
• Gene Symbol: NSBP1
• Gene ID (NCBI): 79366
• RRID: AB_2879374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: P82970
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NSBP1, also named Nucleosome-binding protein 1 or HMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596).