NSBP1 Polyclonal antibody

NSBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23955-1-AP

Synonyms

Nucleosome-binding protein 1, NSBP 1, HMGN5, High mobility group nucleosome-binding domain-containing protein 5

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Validation Data Gallery

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  WB analysis of PC-3 using 23955-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23955-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23955-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23955-1-AP (NSBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 23955-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human testis using 23955-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 23955-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 23955-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human testis using 23955-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human testis using 23955-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23955-1-AP (NSBP1 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IF Staining of HepG2 using 23955-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSBP1 antibody (23955-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

23955-1-AP targets NSBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NSBP1 fusion protein Ag20884
• Full Name: nucleosomal binding protein 1
• Calculated Molecular Weight: 282 aa, 32 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005342
• Gene Symbol: NSBP1
• Gene ID (NCBI): 79366
• RRID: AB_2879374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NSBP1, also named Nucleosome-binding protein 1 or HMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596).

Publications

Species	Application	Title
human	WB	Eur Rev Med Pharmacol Sci; HMGN5 promotes invasion and migration of colorectal cancer through activating FGF/FGFR pathway.; Authors - G-J Zhu