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  • KD/KO Validated

Neurotrophin 4 Polyclonal antibody

Neurotrophin 4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12297-1-AP

Synonyms

neurotrophin 4, Neurotrophin 5, Neutrophic factor 4, NT 4, NT 4/5, NT 5, NT4, NT-4, NT5, NTF4, NTF5



Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, rat brain tissue, rat cerebellum tissue, rat kidney tissue
Positive IF detected inPC-3 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12297-1-AP targets Neurotrophin 4 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neurotrophin 4 fusion protein Ag2938
Full Name neurotrophin 4
Calculated Molecular Weight 210 aa, 22 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC012421
Gene Symbol Neurotrophin 4
Gene ID (NCBI) 4909
RRIDAB_10598309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).

Protocols

Product Specific Protocols
WB protocol for Neurotrophin 4 antibody 12297-1-APDownload protocol
IF protocol for Neurotrophin 4 antibody 12297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

Int J Oncol

Upregulated NTF4 in colorectal cancer promotes tumor development via regulating autophagy.

Authors - Zhou Yang
  • KD Validated
humanWB

Onco Targets Ther

Analysis of risk factors for colon cancer progression.

Authors - Zhou Yang