CoraLite® Plus 488-conjugated Neurotrophin 4 Polyclonal antibody

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  IF Staining of PC-3 using CL488-12297

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 Neurotrophin 4 antibody (CL488-12297) at dilution of 1:200.

• Featured Product
• KD/KO Validated

Neurotrophin 4 Polyclonal Antibody for IF/ICC

Cat No. CL488-12297

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

neurotrophin 4, Neurotrophin 5, Neutrophic factor 4, NT 4, NT 4/5, NT 5, NT4, NT-4, NT5, NTF4, NTF5

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Tested Applications

• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12297 targets Neurotrophin 4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2938

  Product name: Recombinant human NTF4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-210 aa of BC012421

  Sequence: MLPLPSCSLPILLLFLLPSVPIESQPPPSTLPPFLAPEWDLLSPRVVLSRGAPAGPPLLFLLEAGAFRESAGAPANRSRRGVSETAPASRRGELAVCDAVSGWVTDRRTAVDLRGREVEVLGEVPAAGGSPLRQYFFETRCKADNAEEGGPGAGGGGCRGVDRRHWVSECKAKQSYVRALTADAQGRVGWRWIRIDTACVCTLLSRTGRA

• Full Name: neurotrophin 4
• Calculated Molecular Weight: 210 aa, 22 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC012421
• Gene Symbol: Neurotrophin 4
• Gene ID (NCBI): 4909
• RRID: AB_3672534
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P34130
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Neurotrophin 4 (NT-4) belongs to the family of neurotrophic factors, and it interacts with the tyrosine kinase B (trkB) receptor. NT-4 has neuroprotective effects following cerebral ischemia. Its role might be similar to brain-derived neurotrophic factor (BDNF) because both interact with trkB. Exercise also improves neural function by increasing neurotrophic factors. NT-4 exists in two forms, either as a dimer (80 kDa) or as a monomer (40-47 kDa) (PMID: 23526925).