Nesprin 2 Polyclonal antibody, PBS Only

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  WB analysis of HEK-293 using 25265-1-AP (same clone as 25265-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25265-1-AP (SYNE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 25265-1-AP (same clone as 25265-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 25265-1-AP (same clone as 25265-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Nesprin 2 antibody (25265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

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  IF Staining of Human dorsal cerebral organoid using 25265-1-AP (same clone as 25265-1-PBS)

  Human dorsal cerebral organoid at 12 weeks. 4% PFA fixed. Antibody dilution was 1:500 for Syne-2 (25265-1-AP; white), DAPI 1:1000. Image created by Wimmer Ryszard from the Institut Curie in Paris, France This data was developed using the same antibody clone with 25265-1-PBS in a different storage buffer formulation.

Nesprin 2 Polyclonal Antibody for WB, IF, Indirect ELISA

Cat No. 25265-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, Indirect ELISA

Synonyms

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Product Information

25265-1-PBS targets Nesprin 2 in WB, IF, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: spectrin repeat containing, nuclear envelope 2
• Calculated Molecular Weight: 799 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: NM_015180
• Gene Symbol: Nesprin 2
• Gene ID (NCBI): 23224
• RRID: AB_2879995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WXH0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.