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Occludin Polyclonal antibody

Occludin Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 13409-1-AP


occludin, OCLN

Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue, human kidney tissue, human liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:1000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:800
Sample-dependent, check data in validation data gallery

Product Information

13409-1-AP targets Occludin in WB, IP, IHC, FC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Occludin fusion protein Ag4057
Full Name occludin
Calculated molecular weight 522 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC029886
Gene symbol OCLN
Gene ID (NCBI) 4950
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).


Product Specific Protocols
WB protocol for Occludin antibody 13409-1-APDownload protocol
IHC protocol for Occludin antibody 13409-1-APDownload protocol
IP protocol for Occludin antibody 13409-1-APDownload protocol
FC protocol for Occludin antibody 13409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets.

Authors - Shiqiong Wang

Lab Invest

Glucagon-like peptide-1 receptor activation alleviates lipopolysaccharide-induced acute lung injury in mice via maintenance of endothelial barrier function.

Authors - Jian Xu

Nat Commun

Intestinal non-canonical NFκB signaling shapes the local and systemic immune response.

Authors - Sadeesh K Ramakrishnan

Front Microbiol

A Novel Postbiotic From Lactobacillus rhamnosus GG With a Beneficial Effect on Intestinal Barrier Function.

Authors - Jie Gao

Biochem Biophys Res Commun

Bacterial β-glucuronidase alleviates dextran sulfate sodium-induced colitis in mice: A possible crucial new diagnostic and therapeutic target for inflammatory bowel disease.

Authors - Yan He

Ren Fail

Cadmium regulates von Willebrand factor and occludin expression in glomerular endothelial cells of mice in a TNF-α-dependent manner.

Authors - Zongguo Sun


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (09-21-2021)

Gave decent results with IHC at a concentration of 1:300-1:400 for mouse intestinal tissue

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:300-1:400
  • Cell Tissue Type: Mouse intestinal tissue

Amy (Verified Customer) (05-22-2019)

I approached Proteintech for guidance with regards to dilutions of this antibody, and with their help I optimized the protocol in good time. Very helpful!

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse Colon

JIMMY (Verified Customer) (03-26-2019)

This antibody offers a good detection of Occludin on mouse brain endothelial cell line, bEnd3 cells, through IHC.

  • Applications: Immunofluorescence,