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  • KD/KO Validated

Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IF, FC, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 22952-1-AP

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, C2C12 cell, HepG2 cells, mouse kidney tissue
Positive IHC detected inmouse kidney tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22952-1-AP targets Osteopontin in WB, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, rabbit, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteopontin fusion protein Ag19216
Full Name secreted phosphoprotein 1
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 70 kDa, 44-66 kDa
GenBank Accession NumberBC007016
Gene Symbol SPP1
Gene ID (NCBI) 6696
RRIDAB_2783651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands corresponding to peptide fragments (PMID: 8195113; 17890765).

Protocols

Product Specific Protocols
WB protocol for Osteopontin antibody 22952-1-APDownload protocol
IHC protocol for Osteopontin antibody 22952-1-APDownload protocol
IF protocol for Osteopontin antibody 22952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Adv Mater

Realizing Highly Efficient Sonodynamic Bactericidal Capability through the Phonon-Electron Coupling Effect Using Two-Dimensional Catalytic Planar Defects

Authors - Congyang Mao
humanIF

Cell Stem Cell

Decoding the temporal and regional specification of microglia in the developing human brain.

Authors - Yanxin Li
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
mouseIF

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian
mouseWB

Biomaterials

A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration

Authors - Congcong Yu
mouseWB

Cell Death Differ

Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis

Authors - Qing-Xin Ji

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (10-26-2023)

The WB bands exhibit remarkable strength and clarity, with various treatments leading to significant changes in expression.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Lung cancer cell lines and lung cancer tissues with different treatment
Osteopontin Antibody Western Blot validation (1:1000 dilution) in Lung cancer cell lines and lung cancer tissues with different treatment (Cat no:22952-1-AP)
FH

Shinford (Verified Customer) (09-24-2023)

This antibody works well in esophageal cancer and lung cancer cell lines, and the detection antibody band is clear. It is recommended to use a diluted concentration of 1:2000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cancer cell lines
Osteopontin Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:22952-1-AP)
FH

Elisa (Verified Customer) (08-16-2023)

Tested on mouse skull sections, with appropriate blocking: give a nice stain of osteopontin, but maybe it can be optimized to be able to use an higher dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse skull
FH

Davide (Verified Customer) (09-20-2022)

The antibody works really well. Fantastic product, and the possibility to try the product with the free sample is something really appreciated. Will surely buy a full vial soon.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Cholangiocytes
Osteopontin Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocytes (Cat no:22952-1-AP)
FH

WEI (Verified Customer) (03-08-2022)

Strong specific Bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart
FH

LUNFENG (Verified Customer) (01-27-2020)

VERY GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Works with one specific band and one faint non-specific band. However, can be distinguished by protein size. So works fine in general.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney
FH

Jingwen (Verified Customer) (01-17-2020)

This antibody showed the specific band and staining results in both western blot and immunofluorescence staining. I strongly recommend it.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Mouse heart and cultured cardiomyocytes.
FH

Barbara (Verified Customer) (09-28-2019)

Fluorescence-based detection of OPN (green) expression in paraffin-embedded human aortic tissue, heat mediated antigen retrival with 10mM citrate buffer (pH 6,0)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: aorta
Osteopontin Antibody Immunohistochemistry, validation (1:150 dilution) in aorta (Cat no:22952-1-AP)