Osteopontin Polyclonal antibody

Osteopontin Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 22952-1-AP

Synonyms

BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, C2C12 cell, HepG2 cells, mouse kidney tissue
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22952-1-AP is Osteopontin Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Osteopontin fusion protein Ag19216
Full Name secreted phosphoprotein 1
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 70 kDa, 44-66 kDa
GenBank accession numberBC007016
Gene symbol SPP1
Gene ID (NCBI) 6696
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues (PMID: 15138464; 16406521). OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. The very acidic nature of OPN, as well as the presence of variable posttranslational modifications, has led to anomalous migration in SDS-polyacrylamide gels and therefore to reports of different molecular weights for OPN (PMID: 8293561). Depending on the cell and tissue source and/or the SDS-PAGE system, OPN migrates with a molecular weight of 44-80 kDa, as well as at some smaller bands correspond to peptide fragments (PMID: 8195113; 17890765).

Protocols

Product Specific Protocols
WB protocol for Osteopontin antibody 22952-1-APDownload protocol
IHC protocol for Osteopontin antibody 22952-1-APDownload protocol
IF protocol for Osteopontin antibody 22952-1-APDownload protocol
FC protocol for Osteopontin antibody 22952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Cell Physiol

Efficient osteogenic differentiation of the dental pulp stem cells on β-glycerophosphate loaded polycaprolactone/polyethylene oxide blend nanofibers.

Authors - Fatemeh Sadat Hosseini
humanIHC

Int Heart J

Osteopontin in the Pathogenesis of Aortic Dissection by the Enhancement of MMP Expressions.

Authors - Fudong Fan
ratWB

Antioxid Redox Signal

Peroxisome proliferator-activated receptor-γ coactivator-1α inhibits vascular calcification through sirtuin 3‒mediated reduction of mitochondrial oxidative stress.

Authors - Han Feng
mouseWB

J Mech Behav Biomed Mater

Citric acid enhances the physical properties, cytocompatibility and osteogenesis of magnesium calcium phosphate cement.

Authors - Shuang Wang
ratWB

Front Pharmacol

Icariin Protects Bone Marrow Mesenchymal Stem Cells Against Iron Overload Induced Dysfunction Through Mitochondrial Fusion and Fission, PI3K/AKT/mTOR and MAPK Pathways.

Authors - Xudong Yao
ratWB

Exp Ther Med

Effect of microRNA-21 on hypoxia-inducible factor-1α in orthodontic tooth movement and human periodontal ligament cells under hypoxia.

Authors - Xueqin Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

LUNFENG (Verified Customer) (01-27-2020)

VERY GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jie (Verified Customer) (01-23-2020)

Works with one specific band and one faint non-specific band. However, can be distinguished by protein size. So works fine in general.

  • Applications: Western Blot,Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney
FH

Jingwen (Verified Customer) (01-17-2020)

This antibody showed the specific band and staining results in both western blot and immunofluorescence staining. I strongly recommend it.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 for WB, 1:100 for IF
  • Cell Tissue Type: Mouse heart and cultured cardiomyocytes.
FH

Barbara (Verified Customer) (09-28-2019)

Fluorescence-based detection of OPN (green) expression in paraffin-embedded human aortic tissue, heat mediated antigen retrival with 10mM citrate buffer (pH 6,0)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: aorta
Osteopontin Antibody Immunohistochemistry, validation (1:150 dilution) in aorta (Cat no:22952-1-AP)