Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells, NCCIT cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat eye tissue |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10837-1-AP targets OPTN/Optineurin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1272 Product name: Recombinant human OPTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-577 aa of BC013876 Sequence: VSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALERKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTVLQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALASKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAERAAREKIHEEKEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRGAEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII Predict reactive species |
| Full Name | optineurin |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC013876 |
| Gene Symbol | OPTN |
| Gene ID (NCBI) | 10133 |
| RRID | AB_2156665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96CV9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
| IF protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
| IHC protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
| IP protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
| WB protocol for OPTN/Optineurin antibody 10837-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
OPTN/Optineurin Polyclonal antibody (Cat# 10837-1-AP) has accumulated 172 citations. Publications appear in journals including Nature, Cell, Nature Communications, Autophagy, Molecular Cell, Neuron, PNAS, EMBO Journal, Cell Death & Differentiation, Science Advances, Cell Reports, and Redox Biology. Key research fields include autophagy and mitophagy, neurodegenerative diseases (ALS, Alzheimer's, glaucoma), viral infections and innate immunity, cancer biology, cardiovascular disease, renal injury, inflammation, and bone metabolism.
| Species | Application | Title |
|---|---|---|
Cell Host Microbe The bacterial effector GarD shields Chlamydia trachomatis inclusions from RNF213-mediated ubiquitylation and destruction | ||
Nat Cell Biol Lysine-11 ubiquitination drives type-I/III interferon induction by cGAS-STING and Toll-like receptors 3 and 4 | ||
Nat Cell Biol The Troyer syndrome protein spartin mediates selective autophagy of lipid droplets |
Reviews
What customers say
The single review for 10837-1-AP is a 5-star rating from a Western Blot application. The user reported the antibody performed very well when incubated overnight at 4°C, using a 1:5000 dilution on HeLa cells. Overall, the feedback is positive, highlighting reliable performance under standard overnight cold incubation conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Muhammed Talha (Verified Customer) (08-31-2023) | It worked very well at 4 degree for overnight.
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