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OPTN Polyclonal antibody
OPTN Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Cat no : 10837-1-AP
|Positive WB detected in||HeLa cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10837-1-AP targets OPTN in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OPTN fusion protein Ag1272|
|Calculated molecular weight||66 kDa|
|Observed molecular weight||66-70 kDa|
|GenBank accession number||BC013876|
|Gene ID (NCBI)||10133|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.
The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.
NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.
TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1
p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS.
Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.
Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Muhammed Talha (Verified Customer) (08-31-2023)
It worked very well at 4 degree for overnight.