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  • KD/KO Validated

OPTN Polyclonal antibody

OPTN Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10837-1-AP

Synonyms

E3 14.7K interacting protein, FIP 2, FIP2, GLC1E, HIP 7, HIP7, Huntingtin yeast partner L, HYPL, NEMO related protein, NRP, optineurin, OPTN, TFIIIA INTP



Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10837-1-AP targets OPTN in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPTN fusion protein Ag1272
Full Name optineurin
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC013876
Gene Symbol OPTN
Gene ID (NCBI) 10133
RRIDAB_2156665
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are the cause of primary open angle glaucoma type 1E (GLC1E). Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for OPTN antibody 10837-1-APDownload protocol
IHC protocol for OPTN antibody 10837-1-APDownload protocol
IP protocol for OPTN antibody 10837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.

Authors - Michael Lazarou
mouseWB

Cell

NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.

Authors - Zhenyu Zhong
humanCoIP

Mol Cell

TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1

Authors - François Le Guerroué
humanIF

Acta Neuropathol

p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS.

Authors - Safa Al-Sarraj
humanWB

Mol Cell

Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.

Authors - Shireen A Sarraf
  • KO Validated
humanWB

Mol Cell

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Authors - Susanne Zellner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Muhammed Talha (Verified Customer) (08-31-2023)

It worked very well at 4 degree for overnight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: hela
OPTN Antibody Western Blot validation (1:5000 dilution) in hela (Cat no:10837-1-AP)