OPTN/Optineurin Polyclonal antibody

• 

  WB analysis using 10837-1-AP

  various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10837-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10837-1-AP (OPTN/Optineurin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10837-1-AP

  WB result of OPTN antibody (10837-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OPTN transfected HeLa cells.

• 

  IP experiment of mouse brain using 10837-1-AP

  IP result of anti-OPTN (IP:10837-1-AP, 3ug; Detection:10837-1-AP 1:800) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human gliomas using 10837-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human gliomas using 10837-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10837-1-AP (OPTN Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of rat eye using 10837-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using OPTN antibody (10837-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 10837-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using OPTN antibody (10837-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  FC experiment of HeLa using 10837-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human OPTN (10837-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

OPTN/Optineurin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 10837-1-AP

Publications(114)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Synonyms

Optineurin, OPTN, ALS12, E3-14.7K-interacting protein, FAA4

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells, NCCIT cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat eye tissue
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 14 publications below
• WB: See 94 publications below
• IHC: See 6 publications below
• IF: See 21 publications below
• IP: See 7 publications below
• CoIP: See 3 publications below

Product Information

10837-1-AP targets OPTN/Optineurin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OPTN/Optineurin fusion protein Ag1272
• Full Name: optineurin
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC013876
• Gene Symbol: OPTN
• Gene ID (NCBI): 10133
• RRID: AB_2156665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96CV9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPTN, also named as FIP2, GLC1E, HIP7, HYPL and NRP, plays a neuroprotective role in the eye and optic nerve. It is probably part of the TNF-alpha signaling pathway that can shift the equilibrium toward induction of cell death. OPTN may act by regulating membrane trafficking and cellular morphogenesis via a complex that contains Rab8 and hungtingtin (HD). OPTN may constitute a cellular target for adenovirus E3 14.7, an inhibitor of TNF-alpha functions, thereby affecting cell death. Defects in OPTN are a cause of susceptibility to normal pressure glaucoma (NPG). OPTN mutated in adult-onset primary open angle glaucoma. It supports the protective role of OPTN in the trabecular meshwork. OPTN has 3 isoforms with MW 66,65 and 60 kDa (refer to UniProt). Catalog#10837-1-AP recognises 66 and 70-74 kDa band, and the additional 70-74 kDa band due to phosphorylation.

Publications

Species	Application	Title
human	WB	Nature; The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy.; Authors - Michael Lazarou
mouse	WB	Cell; NF-κB Restricts Inflammasome Activation via Elimination of Damaged Mitochondria.; Authors - Zhenyu Zhong
human	CoIP	Mol Cell; TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1; Authors - François Le Guerroué
human	IF	Acta Neuropathol; p62 positive, TDP-43 negative, neuronal cytoplasmic and intranuclear inclusions in the cerebellum and hippocampus define the pathology of C9orf72-linked FTLD and MND/ALS.; Authors - Safa Al-Sarraj
human	WB	Mol Cell; Loss of TAX1BP1-Directed Autophagy Results in Protein Aggregate Accumulation in the Brain.; Authors - Shireen A Sarraf ; KO Validated
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Muhammed Talha (Verified Customer) (08-31-2023)

It worked very well at 4 degree for overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: hela

OPTN/Optineurin Antibody Western Blot validation (1:5000 dilution) in hela (Cat no:10837-1-AP)