ORC3L Polyclonal antibody

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  WB analysis using 32429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32429-1-AP (ORC3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ORC3L Polyclonal Antibody for WB, ELISA

Cat No. 32429-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

LATHEO, ORC3, Origin recognition complex subunit 3, Origin recognition complex subunit Latheo

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32429-1-AP targets ORC3L in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORC3L fusion protein Ag37481
• Full Name: origin recognition complex, subunit 3-like (yeast)
• Calculated Molecular Weight: 66 kDa, 82 kDa
• Observed Molecular Weight: 66 kDa, 82 kDa
• GenBank Accession Number: NM_012381.3
• Gene Symbol: ORC3L
• Gene ID (NCBI): 23595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9UBD5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ORC3L, also known as Origin Recognition Complex Subunit 3-like, is a human gene that encodes a protein which is a subunit of the Origin Recognition Complex (ORC). The ORC is a highly conserved six-subunit protein complex that is essential for the initiation of DNA replication in eukaryotic cells. Studies in yeast have shown that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins.