OTX2 Polyclonal antibody

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  WB analysis using 13497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13497-1-AP (OTX2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 13497-1-AP

  IP result of anti-OTX2 (IP:13497-1-AP, 3ug; Detection:13497-1-AP 1:1500) with Y79 cells lysate 2400ug.

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  IP experiment of Y79 using 13497-1-AP

  IP result of anti-OTX2 (IP:13497-1-AP, 4ug; Detection:13497-1-AP 1:2000) with Y79 cells lysate 2000ug.

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  IHC staining of human gliomas using 13497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 13497-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13497-1-AP (OTX2 antibody) at dilution of 1:100 (under 10x lens).

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  FC experiment of HepG2 using 13497-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human OTX2 (13497-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

OTX2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 13497-1-AP

Publications(33)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, FC (Intra), IP, CoIP, ChIP, ELISA

Synonyms

MCOPS5, Homeobox protein OTX2

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Tested Applications

• Positive WB detected in: Y79 cells, mouse retina tissue, rat retina tissue
• Positive IP detected in: Y79 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 7 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 7 publications below

Product Information

13497-1-AP targets OTX2 in WB, IHC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OTX2 fusion protein Ag4323
• Full Name: orthodenticle homeobox 2
• Calculated Molecular Weight: 297 aa, 32 kDa
• Observed Molecular Weight: 31-35 kDa
• GenBank Accession Number: BC032579
• Gene Symbol: OTX2
• Gene ID (NCBI): 5015
• RRID: AB_2157176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P32243
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; Unified rhombic lip origins of group 3 and group 4 medulloblastoma; Authors - Kyle S. Smith
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	WB	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du ; KD Validated
mouse	IF,ChIP	Science; Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.; Authors - Catherine J Peña
human	chip	Cancer Cell; Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.; Authors - Pascal D Johann
		Cell Rep; ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells; Authors - Danping Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

David (Verified Customer) (02-21-2022)

This antibody works very well for immunofluorescence on human brain.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: Human brain

OTX2 Antibody Immunofluorescence validation (1 to 200 dilution) in Human brain (Cat no:13497-1-AP)