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  • KD/KO Validated

OTX2 Polyclonal antibody

OTX2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 13497-1-AP


Homeobox protein OTX2, MCOPS5, orthodenticle homeobox 2, Orthodenticle homolog 2, OTX2

Tested Applications

Positive WB detected inY79 cells, mouse retina tissue, rat retina tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse embryo tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13497-1-AP targets OTX2 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTX2 fusion protein Ag4323
Full Name orthodenticle homeobox 2
Calculated Molecular Weight 297 aa, 32 kDa
Observed Molecular Weight 31-35 kDa
GenBank Accession NumberBC032579
Gene Symbol OTX2
Gene ID (NCBI) 5015
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive


Product Specific Protocols
WB protocol for OTX2 antibody 13497-1-APDownload protocol
IHC protocol for OTX2 antibody 13497-1-APDownload protocol
IF protocol for OTX2 antibody 13497-1-APDownload protocol
IP protocol for OTX2 antibody 13497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Unified rhombic lip origins of group 3 and group 4 medulloblastoma

Authors - Kyle S. Smith


Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
  • KD Validated


Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.

Authors - Catherine J Peña

Cancer Cell

Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.

Authors - Pascal D Johann

Cell Rep

ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells

Authors - Danping Lu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


David (Verified Customer) (02-21-2022)

This antibody works very well for immunofluorescence on human brain.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: Human brain
OTX2 Antibody Immunofluorescence validation (1 to 200 dilution) in Human brain (Cat no:13497-1-AP)