- Featured Product
- KD/KO Validated
OTX2 Polyclonal antibody
OTX2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, ChIP,ELISA
Conjugate
Unconjugated
Cat no : 13497-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse embryo tissue |
| Positive FC detected in | hESC cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| ChIP | See 5 publications below |
Product Information
13497-1-AP targets OTX2 in WB, IP, IHC, IF, FC, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OTX2 fusion protein Ag4323 |
| Full Name | orthodenticle homeobox 2 |
| Calculated molecular weight | 297 aa, 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC032579 |
| Gene symbol | OTX2 |
| Gene ID (NCBI) | 5015 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OTX2 antibody 13497-1-AP | Download protocol |
| IHC protocol for OTX2 antibody 13497-1-AP | Download protocol |
| IF protocol for OTX2 antibody 13497-1-AP | Download protocol |
| IP protocol for OTX2 antibody 13497-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett DMBX1 promotes tumor proliferation and regulates cell cycle progression via repressing OTX2-mediated transcription of p21 in lung adenocarcinoma cell. | ||
Cancer Manag Res Effective Inhibition of MYC-Amplified Group 3 Medulloblastoma Through Targeting EIF4A1. | ||
Dev Dyn OTX2 regulates CFTR expression during endoderm differentiation and occupies 3' cis-regulatory elements.
| ||
Neuropharmacology Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex. | ||
RNA Biol Molecular network of miR-1343 regulates the pluripotency of porcine pluripotent stem cells via repressing OTX2 expression. | ||
Stem Cell Reports Human Clinical-Grade Parthenogenetic ESC-Derived Dopaminergic Neurons Recover Locomotive Defects of Nonhuman Primate Models of Parkinson's Disease. |
