|Positive WB detected in||HUVEC cells, Jurkat cells, mouse colon tissue, rat colon tissue, 4T1 cells, pig colon tissue|
|Positive IHC detected in||human colon cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Caco-2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:800-1:3200|
|Sample-dependent, check data in validation data gallery|
66378-1-Ig targets Occludin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Occludin fusion protein Ag4057|
|Calculated molecular weight||522 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC029886|
|Gene ID (NCBI)||4950|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Doriane (Verified Customer) (08-25-2019)
Satisfying antibody used for automated Western-blot Wes (Protein Simple)