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Occludin Monoclonal antibody
Occludin Monoclonal Antibody for IF, IHC, WB,ELISA
Cat no : 66378-1-Ig
Validation Data Gallery
|Positive WB detected in||HUVEC cells, Jurkat cells, mouse colon tissue, rat colon tissue, 4T1 cells, pig colon tissue|
|Positive IHC detected in||human colon cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Caco-2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:800-1:3200|
|Sample-dependent, check data in validation data gallery|
66378-1-Ig targets Occludin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat, bovine, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Occludin fusion protein Ag4057|
|Calculated molecular weight||522 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC029886|
|Gene ID (NCBI)||4950|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
ACS Pharmacol Transl Sci
Extending the Calpain-Cathepsin Hypothesis to the Neurovasculature: Protection of Brain Endothelial Cells and Mice from Neurotrauma.
J Anim Physiol Anim Nutr (Berl)
Seaweed polysaccharide mitigates intestinal barrier dysfunction induced by enterotoxigenic Escherichia coli through NF-κB pathway suppression in porcine intestinal epithelial cells.
J Anim Sci
Effects and interaction of dietary electrolyte balance and citric acid on the intestinal function of weaned piglets.
The ameliorative effect of melatonin on LPS-induced Sertoli cells inflammatory and tight junctions damage via suppression of the TLR4/MyD88/NF-κB signaling pathway in newborn calf.
Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.
Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Doriane (Verified Customer) (08-25-2019)
Satisfying antibody used for automated Western-blot Wes (Protein Simple)