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Occludin Monoclonal antibody
Occludin Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66378-1-Ig
Validation Data Gallery
|Positive WB detected in
|HUVEC cells, Jurkat cells, mouse colon tissue, LNCaP cells, U2OS cells, pig colon tissue, rat colon tissue, 4T1 cells, HEK-293 cells
|Positive IHC detected in
|human colon cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Caco-2 cells, MCF-7 cells
|Western Blot (WB)
|WB : 1:5000-1:50000
|IHC : 1:50-1:500
|IF : 1:800-1:3200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66378-1-Ig targets Occludin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|human, mouse, rat, pig
|human, mouse, rat, sheep, pig, bovine
|Host / Isotype
|Mouse / IgG1
|Occludin fusion protein Ag4057
|Calculated molecular weight
|522 aa, 59 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).
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Shensong Yangxin Capsule Reduces the Susceptibility of Arrhythmia in db/db Mice via Inhibiting the Inflammatory Response Induced by Endothelium Dysfunction
J Am Soc Nephrol
Close Association Between Altered Urine-urothelium Barrier and Tertiary Lymphoid Structure Formation in the Renal Pelvis During Nephritis.
J Mol Cell Biol
NRF1-mediated microglial activation triggers high-altitude cerebral edema.
Bacterial butyrate mediates the anti-atherosclerotic effect of silybin
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Saba (Verified Customer) (07-25-2022)
The quality of the band is very good and you can easily find the specific band on your membrane.
Doriane (Verified Customer) (08-25-2019)
Satisfying antibody used for automated Western-blot Wes (Protein Simple)