• Featured Product
  • KD/KO Validated

Occludin Monoclonal antibody

Occludin Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, rat, pig and More (2)







Cat no : 66378-1-Ig


Occludin, OCLN

Tested Applications

Positive WB detected inHUVEC cells, Jurkat cells, mouse colon tissue, LNCaP cells, U2OS cells, pig colon tissue, rat colon tissue, 4T1 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells, MCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66378-1-Ig targets Occludin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, sheep, pig, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Occludin fusion protein Ag4057
Full Name occludin
Calculated Molecular Weight 522 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC029886
Gene Symbol Occludin
Gene ID (NCBI) 4950
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).


Product Specific Protocols
WB protocol for Occludin antibody 66378-1-IgDownload protocol
IHC protocol for Occludin antibody 66378-1-IgDownload protocol
IF protocol for Occludin antibody 66378-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Circ Res

Endothelial BACE1 Impairs Cerebral Small Vessels via Tight Junctions and eNOS.

Authors - Haoyue Zhou

Sci Adv

Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage

Authors - Dayun Feng

Drug Des Devel Ther

Shensong Yangxin Capsule Reduces the Susceptibility of Arrhythmia in db/db Mice via Inhibiting the Inflammatory Response Induced by Endothelium Dysfunction

Authors - Jiehan Zhang

J Am Soc Nephrol

Close Association Between Altered Urine-urothelium Barrier and Tertiary Lymphoid Structure Formation in the Renal Pelvis During Nephritis.

Authors - Osamu Ichii

J Mol Cell Biol

NRF1-mediated microglial activation triggers high-altitude cerebral edema.

Authors - Xueting Wang


Gut microbiota-derived gamma-aminobutyric acid from metformin treatment reduces hepatic ischemia/reperfusion injury through inhibiting ferroptosis

Authors - Fangyan Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Saba (Verified Customer) (07-25-2022)

The quality of the band is very good and you can easily find the specific band on your membrane.

  • Applications: Western Blot

Doriane (Verified Customer) (08-25-2019)

Satisfying antibody used for automated Western-blot Wes (Protein Simple)

  • Applications: Western Blot, Other,
  • Primary Antibody Dilution: Automated Western Blot Wes 1/400
  • Cell Tissue Type: Caco-2/TC7 cell line